FastQCFastQC Report
Tue 21 Jun 2016
000000000-AP3VM_l01n01.33100000058c58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3VM_l01n01.33100000058c58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15629972
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA336438421.525208106578823No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA177383311.348919882901901No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA9824556.285711836208025No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6759404.324639864997839No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA5331353.410978599321867No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA3405062.178545169498704No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3186812.038909602653159No Hit
AACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2545571.6286465516380961No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2418821.5475523564597555No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGCGCGCGTA2095991.3410068808824482No Hit
TACAGAGGGTGCAAGCGTTAATCAGAATGACTGGGCGTAAAGGGCGTGTA2039211.3046792406281982No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1945031.2444232145777356No Hit
TACGGAGGATGCGAGCGTTATTCGGATTTATTGGGTTTAAAGGGTGCGTA1621731.0375770346869464No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1511910.9673145927580676No Hit
TACGGAGGGTCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1396100.8932197703233249No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1375630.8801231377765744No Hit
TACGAGATGTGCGAGCGTTGTTCGGAATCACTGGGCATAAAGGGTGCGTA1301470.8326758358876138No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATGACTGGGCGTAAAGGGCGTGTA1204700.770762737130943No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCATGTA1185970.7587793503404869No Hit
TACGGTGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1140140.7294574807939516No Hit
AACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA990840.6339358765326003No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA948740.6070004476015696No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA831540.5320163081546148No Hit
TACGGAGGGTCTAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA819240.5241468122911545No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA789440.505080879223584No Hit
TACGGGGGGTCCAAGCGTTATTCGGAATCATTGGGCGTAAAGCGCATGTA788260.5043259194578211No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA699050.4472496815733259No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA656700.4201543035393793No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA656190.419828007369431No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATCATTGGGCGTAAAGAGCGCGTA633250.4051510776858718No Hit
TACGGTGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA603390.38604675683360146No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA594330.3802502013439307No Hit
TACGGAGGATCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA577630.36956560126915133No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA540430.34576517475527146No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA521030.33335312436900083No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA508830.32554760814670686No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA504830.3229884225000531No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA495870.3172558466515487No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA423430.27090899459064927No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA400280.2560977076606407No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA398860.25518919675607865No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA340730.21799783134608303No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA337600.21599526857757648No Hit
TACGGAGGGTCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA327160.20931579403981018No Hit
TACGGTGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA324870.20785066025710092No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCA323870.2072108638454375No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA306780.19627674317010932No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA299430.19157423954438307No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA288100.18432534620023633No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGAGTACGTA280670.17957165886157697No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGAGTA271530.17372391965897316No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA254580.1628793704812779No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA229640.14692284797439176No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA222310.14223314027689876No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA209290.1339029909970408No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA208420.1333463681188936No Hit
TACGGAGGATACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA201180.12871424209845034No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA196040.12542568854250027No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA193300.12367264637454245No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA189100.12098550144555602No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA177680.11367902642435955No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA163000.1042868151011403No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA158340.10130536382278868No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA157920.10103664932989004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACACA3887900.0244.820397
AGGATCC1089500.0244.763896
ACACAAG3884500.0244.744559
GACACAA3884000.0244.678288
GGAGGAT3962200.0244.536254
ATGCGAG2442650.0244.453359
AGGACAC3766250.0244.388526
AGGATGC2836900.0244.287496
TACGGAG10269200.0244.280691
GGATCCG260500.0244.200587
GATCCGA260250.0244.05868
CGGAGGG2512850.0243.951863
AACGTAG407700.0243.888261
GTCCAAG328950.0243.808329
GAGGACA3773550.0243.656055
GGATGCG2458050.0243.420187
ATCCGAG260950.0243.356959
GGAGGAC3805100.0243.251884
ACGGAGG10327250.0242.864852
TACGAGA159250.0242.846131
Produced by FastQC (version 0.11.2)