FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n02_6a_mcb16_adapter2.3520000006fa1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n02_6a_mcb16_adapter2.3520000006fa1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435586
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC66350.0211.582389
TCGGGAG58850.0193.793443
CGGGAGA70050.0191.48814
GATCGGG65250.0189.616741
ATCGGGA67400.0182.295882
GGGAGAG122100.0174.870915
GAGAGGG99700.0168.698497
GGAGAGG101100.0167.574086
GAGCGGC35600.0143.490089
GATTGGG45450.0136.650091
GGAGAGC44650.0128.94736
AAGGGGC29600.0127.879999
TGGGAGA58100.0127.771014
TTGGGAG48700.0126.0214843
GAGAGCG45200.0124.668077
AGAGCGG49450.0124.3578648
ATTGGGA56250.0109.9777152
GAACGGG22000.0108.579481
AGAGGGG173450.0103.7488568
AACGGGA24900.094.9497452
Produced by FastQC (version 0.11.2)