FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n02_10p_mcb16_adapter6.3520000006fa0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n02_10p_mcb16_adapter6.3520000006fa0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856169
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC56150.0197.657979
GATCGGG63550.0175.798511
ATCGGGA63500.0175.551092
TCGGGAG52650.0174.966673
CGGGAGA67100.0174.165334
GGGAGAG110100.0155.211095
GAGAGGG89750.0144.67967
GGAGAGG90500.0144.15746
GAACGGG28500.0129.806951
AAGGGGC29750.0116.117599
GAGCGGC34350.0113.406079
AACGGGA31950.0113.106362
GGAGAGC46400.0103.4913256
GAGAGCG47050.0100.759787
AGAGCGG50000.098.734948
AGAGGGG144650.097.644278
GATTGGG36000.093.576341
ACGGGAG33350.084.482723
CGGGAAA42250.080.893454
TGGGAGA45650.078.357034
Produced by FastQC (version 0.11.2)