FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n02_10l_mcb16_adapter4.3520000006f9ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n02_10l_mcb16_adapter4.3520000006f9ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566688
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC28450.0127.021089
GATCGGG40400.0124.9257351
ATCGGGA41400.0120.428742
TCGGGAG33200.0120.286143
CGGGAGA37500.0109.7599954
GGGAGAG66000.098.371215
GGAGAGG60350.079.975146
GAGAGGG60150.079.222777
AGAGGGG79500.063.330188
GGTCGCG18000.062.61111245
GATTGGG41550.057.7857931
TCGTGTG27450.052.213108245
AAGGGGC25050.051.3473059
GGAGAGC34900.050.8954126
TGGTCGG27800.048.911865245
TTGGGAG39750.047.7672923
TCTCGGG47100.044.474518245
GAGAGCG39500.044.34817
TGGGAGA47350.044.23974
GAGCGGC30850.044.076179
Produced by FastQC (version 0.11.2)