FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n02_10d_mcb16_adapter1.3520000006f9e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n02_10d_mcb16_adapter1.3520000006f9e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555504
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC54450.0179.981649
CGGGAGA56850.0153.852234
TCGGGAG38600.0140.272023
GGGAGAG106700.0134.440025
GAACGGG33600.0131.250021
GGAGAGG89950.0131.011676
ATCGGGA46550.0130.789472
GATCGGG47850.0129.284231
GAGAGGG94400.0124.186977
AACGGGA40150.0107.397262
GAGCGGC40050.0100.6304559
GGAGAGC47700.099.129986
ACGGGAG38800.093.1378863
AGAGCGG54600.092.884618
AGAGGGG140800.092.0498
GAGAGCG51150.090.0488747
CGGAGAG33150.088.318255
AAGGGGC31900.081.794679
TGGGAGA55300.079.5253144
GATTGGG42500.072.635291
Produced by FastQC (version 0.11.2)