FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n01_6a_mcb16_adapter2.3510000006fa1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n01_6a_mcb16_adapter2.3510000006fa1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435586
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1724137.078912426003433TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC218520.8971968142369023TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATTTCGTATGC26470.10868021084043018TruSeq Adapter, Index 4 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1545250.034.6828239
AGAGCAC1551500.034.6694378
CGGAAGA1585200.034.4424254
TCGGAAG1593450.034.4101683
GAAGAGC1574050.034.359546
GATCGGA1614200.034.1727331
ATCGGAA1621250.034.0241362
AAGAGCA1589700.033.9981657
GGAAGAG1599800.033.990275
GGATCGG11350.028.0616131
GATCGGG15650.014.8721721
GAGCACC17956.202754E-1011.6016469
ATCGGGA19451.9463187E-1011.3367372
CGGAAGG19051.9317667E-810.2886924
CGGAAAG29350.010.0170144
TGCTTGA1129600.09.61041655-59
GAGGCAC14156.660818E-59.5229479
CTGCTTG1152800.09.49564355-59
GCTTGAA1107950.09.28076855-59
TCTGCTT1194250.09.16401655-59
Produced by FastQC (version 0.11.2)