FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n01_10p_mcb16_adapter6.3510000006fa00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n01_10p_mcb16_adapter6.3510000006fa00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856169
Sequences flagged as poor quality0
Sequence length251
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1533395.368694919663367TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC67680.2369607680777993TruSeq Adapter, Index 12 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2029600.021.921569
AGAGCAC2038050.021.8667358
TCGGAAG2082100.021.8159543
CGGAAGA2076850.021.7590354
GATCGGA2104500.021.7001671
GAAGAGC2068550.021.6746036
ATCGGAA2112500.021.6179892
GGAAGAG2092300.021.5163945
AAGAGCA2085750.021.4606277
GGATCGG13651.0186341E-1014.3589741
CGGAAGG22000.012.254
GGAGCAC15801.703047E-710.854439
GAATCGG16302.616107E-710.5214721
GAGGCAC15050.00128607168.1395359
GATCGGG16704.0206383E-48.0688631
CGGAAAG34107.421477E-107.90322544
CCAGTCC22652.244897E-57.5717435245
AAGGAGC26903.7363006E-67.28624537
AGGAGCA32801.8964784E-77.09603648
GAAGCAC31105.917864E-77.09003269
Produced by FastQC (version 0.11.2)