FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n01_10l_mcb16_adapter4.3510000006f9f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n01_10l_mcb16_adapter4.3510000006f9f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566688
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC716982.0102122753658294TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2140700.09.6136779
AGAGCAC2149450.09.585948
TCGGAAG2207500.09.5058893
CGGAAGA2202900.09.5034954
GAAGAGC2195150.09.4756626
GATCGGA2230650.09.4511681
ATCGGAA2244350.09.3989342
GGAAGAG2228250.09.3788835
AAGAGCA2216500.09.3733367
AGTCCAT31552.6252968E-45.8240884
Produced by FastQC (version 0.11.2)