FastQCFastQC Report
Thu 17 Nov 2016
000000000-AP3MR_l01n01_10d_mcb16_adapter1.3510000006f9e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MR_l01n01_10d_mcb16_adapter1.3510000006f9e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555504
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1532744.310893757959491TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2152850.020.7290829
AGAGCAC2165650.020.6461588
TCGGAAG2225050.020.50793
CGGAAGA2213850.020.506524
GAAGAGC2203400.020.4703456
GATCGGA2254200.020.4057331
ATCGGAA2263900.020.2912462
AAGAGCA2224450.020.2325767
GGAAGAG2239500.020.2005395
GGATCGG17600.018.7925531
GGAGCAC21008.726238E-89.3333039
CGGAAAG43750.09.239974
CGGAAGG31401.0715667E-87.8025234
TCGGAAA56650.07.3521393
GATCGGG22500.0013036696.53331231
TCGGGAA32508.859835E-66.4076723
ATGCCGC27250.00172863135.8440175245
AAGGAGC38451.8939185E-55.7347027
TGCTTGA1611400.05.665055855-59
CTGCTTG1626500.05.629031755-59
Produced by FastQC (version 0.11.2)