FastQCFastQC Report
Tue 8 Nov 2016
000000000-AP3MB_l01n03.3330000006e888.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MB_l01n03.3330000006e888.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15326442
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA198996212.983848436577777No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7987515.21158792105826No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG7787995.081407674396967No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA6585944.297109531357637No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG4030502.6297688661203953No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG3556532.320519008912832No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG3161552.0628075322374233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3107822.027750472027363No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG2841591.8540441414908952No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2731591.7822727544984023No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG2569021.676201169195042No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGA2516041.6416334593508397No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC2336381.524411210377464No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT2220541.448829415202824No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1872421.2216925493862176No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1753921.1443751915806684No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA1606461.048162384981459No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1340680.8747496646645059No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1322960.8631879466871698No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1180750.7704005926489658No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1060540.6919675160092603No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1049830.6849795927848094No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG982850.6412773427779259No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG975080.6362076729876379No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA954870.6230213117956536No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA874900.570843513452111No Hit
CCTGTTTAATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA813540.5308081288533895No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA791950.5167213629882265No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC750850.48990496293921315No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA680010.44368418971604756No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG667360.4354304802119109No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA652010.4254151093906857No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC643390.41979084251909216No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA617330.4027875484734161No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA576350.37604944448294No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAA559070.3647748120535738No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATAATGGCCAGAA536160.3498267895445009No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA505790.33001136206302806No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA501340.32710788322560447No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA453640.29598519995704153No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG399390.2605888568266529No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG381790.24910543490785403No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC368730.24058421387038167No Hit
CCTGTTTACTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG329550.21502055075796456No Hit
CCCGTTCACTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA325880.2126259963010332No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG310150.20236268796110668No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA297510.19411550312851475No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG292970.1911533022471882No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA291230.19001800939839789No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG272830.1780126137560172No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA258400.16859751271691106No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA253890.16565488585021884No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA247070.1612050598566843No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG244220.15934552846642425No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA238350.155515546269643No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG223690.1459503777850071No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA219880.14346447792644895No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT216950.1415527491638307No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT210260.1371877439003782No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG209150.1364635053589085No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA205650.13417987031823825No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG193750.12641551117995944No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTGTTATCCCAGGA188230.12281389248724524No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG182210.11888604021729245No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA173830.11341836546277341No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG171430.11185244429202812No Hit
CCTGTTTACTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG171420.11184591962048335No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA168410.10988199348550695No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA154640.10089752076835577No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA153890.10040817040249785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC5895500.0244.74653
CTGTTCG3209450.0244.713752
TGTTCGA522200.0244.460463
TTTGCTC4873750.0244.4425
TGTTTGA2957250.0244.395223
TTTGATC2379000.0244.222475
CCTGTTC3311800.0243.938431
CTGTTTG8880100.0243.631552
GTTTGCT5963400.0243.459354
TTGATAC609000.0243.189656
TGTTCGC2710450.0242.930043
TTGCTCC4931750.0242.901116
CCGTTCG1048750.0242.23172
GTTTGAT2989350.0241.91434
GATCCCC2439050.0241.91128
TGATCCC2417350.0241.564217
CCTGTTT9236200.0241.417661
CTCCCCA6368800.0240.699199
GCTCCCC7572000.0240.259848
GTTCGCT3816950.0239.983754
Produced by FastQC (version 0.11.2)