FastQCFastQC Report
Tue 8 Nov 2016
000000000-AP3MB_l01n02.3320000006e88b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3MB_l01n02.3320000006e88b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15326442
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGTACCCAA4034152.632150371234237No Hit
GAAGAAGCGGTA3402352.219921623035536No Hit
CGACTGTCTTAA3094202.018863869383383No Hit
ATCGCTCGAGGA2941141.9189972467191014No Hit
CTATTTGCGACA2655481.7326134793711416No Hit
ACACGTAAGCCT2572631.6785565756227048No Hit
AGTAGAGGGATG2197591.433855294007572No Hit
TCCTCTGTCGAC2196461.4331180061230127No Hit
CCTCTCGTGATC2159081.4087287838886546No Hit
GACTTGGTATTC2082611.3588346205857824No Hit
CGATCCGTATTA1984801.2950168082063664No Hit
TACACGATCTAC1901561.2407054422676835No Hit
ATGTCGAGAGAA1869271.2196372778496145No Hit
ATTCCTGTGAGT1766431.152537555683178No Hit
TATCAGGTGTGC1707851.1143160297739032No Hit
TAGCTCGTAACT1664461.086005479941137No Hit
TAGGATTGCTCG1659431.0827235701541167No Hit
ATGTGCACGACT1618821.0562268790107971No Hit
TAACACCACATC1608411.0494346959326895No Hit
TACTAATCTGCG1547761.0098625630136466No Hit
ACGCGCAGATAC1544951.0080291303095656No Hit
GCTCAGTGCAGA1538781.0040034079664413No Hit
AAGGAGCGCCTT1530220.9984182891241163No Hit
CGGAGCTATGGT1488310.9710733906799764No Hit
TAATCCACAGCG1478630.9647575086246372No Hit
ATCCCGAATTTG1472720.9609014277416768No Hit
CAGGCGTATTGG1469290.9586634654018199No Hit
ATCGATCTGTGG1448550.9451312966179626No Hit
TGGCAAGACTCT1430300.9332237710487535No Hit
TCGGAGTGTTGT1403370.9156528305786822No Hit
GCACACACGTTA1402130.9148437713071306No Hit
GTTGGTCAATCT1359760.8871987379719312No Hit
GGATCGCAGATC1350650.8812547621946437No Hit
CAGTGCATATGC1291720.8428048727813017No Hit
ACCACATACATC1276480.8328612733470692No Hit
ATTGGGCTAGGC1257890.8207319089453378No Hit
ACTCACGGTATG1253620.8179458741957201No Hit
GACTTTCCCTCG1249990.8155774184249678No Hit
GAGTGGTAGAGA1237270.8072780362200176No Hit
TACAGATGGCTC1230240.8026911921240429No Hit
TCGAGGACTGCA1227730.801053499566305No Hit
ACGCCACGAATG1195610.780096254564497No Hit
AAGAGATGTCGA1185010.7731801027270386No Hit
AGCTGTTGTTTG1154060.7529862442959689No Hit
TCGTCGATAATC1128800.7365049239738747No Hit
GTACCTAATTGC1116390.7284078065868126No Hit
TTAGGGCTCGTA1090320.7113979878695916No Hit
ATGTGGGACCCA1086470.7088859893248544No Hit
ATGAGACTCCAC1080550.7050233837703492No Hit
GCACGACAACAC1078180.7034770366142383No Hit
CCTCGTTCGACT1044200.6813062027051027No Hit
ATACTTCGCAGG1042530.6802165825571258No Hit
GCGGCAATTACG1029060.6714278499863178No Hit
GGATGGTGTTGC1017650.6639831997537328No Hit
TGCAATGTTGCT1014030.6616212686545253No Hit
CTGCTAACGCAA1003950.655044399737395No Hit
TGAGTCACTGGT1001370.6533610344788437No Hit
GCTGATGAGCTG982210.6408597637990605No Hit
GTACATACCGGT979850.6393199413144942No Hit
CAACACGCACGA973200.6349810347372208No Hit
AATCTTGCTGCA967900.6315229588184916No Hit
AGCTTGACAGCT954970.6230865585111013No Hit
GACTCGAATCGT951740.6209790896021399No Hit
TCCGAATTCACA943060.6153156747012777No Hit
AATGCCTCAACT940580.6136975561581742No Hit
TGAGCCGGAATC912810.5955785432783421No Hit
GTATGCGCTGTA893240.5828097610652231No Hit
AACACAAGGAGT885630.5778444860196515No Hit
GGTGTCTATTGT885300.577629171858674No Hit
CGAGAAGAGAAC877620.572618224112289No Hit
TCAAGCTCAAGC877330.5724290086374907No Hit
TCAACAGCATCG870930.5682532188488365No Hit
GACAGGAGATAG868790.5668569391382553No Hit
GAATCTTCGAGC864580.5641100524179062No Hit
GTCCGAAACACT859330.5606845998569009No Hit
GTCTACACACAT840900.5486596301998858No Hit
GTCTAATTCCGA838160.5468718701966184No Hit
GACACATTTCTG819450.5346642097363498No Hit
TGTAATTGTCGC788870.5147117641524367No Hit
GCCTGAATTTAC781920.5101771174288201No Hit
TCCGACACAATT774900.5055967980043901No Hit
AGTTCCCGAGTA758090.4946288251376282No Hit
GCATGGCTCTAA756760.49376104382217345No Hit
CGCAGCGGTATA745710.4865512817652003No Hit
TCACGGGAGTTG743700.4852398227847011No Hit
GGTCACTGACAG731670.47739064291634026No Hit
TAGGAACTGGCC728160.47510048320412523No Hit
TCTGTTGCTCTC693870.4527273844771017No Hit
GTACGTGGGATC684370.44652894650956826No Hit
CCATAGGGTTCA628990.4103953154946203No Hit
AATGGAGCATGA624680.40758318205882355No Hit
CCACCTACTCCA613710.4004256173742086No Hit
CTTGTGTCGATA613200.4000928591254252No Hit
GATTGGTTGCAC600550.3918391496212885No Hit
CACGCCATAATG587390.3832526818683684No Hit
CCATGCGATAAC571500.3728849787837255No Hit
TTATGCAGTCGT567070.3699945492893915No Hit
ACTGACAGCCAT521320.3401441769720591No Hit
TCTTCCGCTACT510100.33282349549882484No Hit
GAATAGAGCCAA492880.32158801109872726No Hit
GAAGTTGGAAGT482800.315011142181597No Hit
TGTTATCGCACA469420.30628113165469195No Hit
ACATTCAGCGCA448170.29241620462205126No Hit
TAACGCTTGGGT339130.22127118609785623No Hit
TATGCACCAGTG262030.17096596848766332No Hit
AATGTCCGTGAC174710.11399253655871337No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGG1451.9645086E-106.00000054
TCGCCGG953.4298118E-66.06
CCTAGGC600.00339655466.06
TTAGCCG600.00339655466.01
CGACCGT550.0091606826.01
GCGCCCT550.0091606826.06
CTCCAGG600.00339655466.05
ACGGGCG1850.06.03
TGGCCAA806.539342E-56.03
GGGACCA550.0091606826.05
CAGATCC909.152351E-66.06
GTGTCCC1950.06.03
AGCTGGC1101.8153332E-76.04
CTACCGG806.539342E-56.05
GGGCCAG650.00126196556.04
GCGGACT1259.677024E-96.03
CTGGGAG704.6973652E-46.05
CGACTGA909.152351E-66.01
CGTTTAC1900.06.02
GCGTACC1800.06.03
Produced by FastQC (version 0.11.2)