FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji8.35200000056f7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji8.35200000056f7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178604
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAG500.001830876573.53
CTTAGGG2351.3242243E-841.70213245
CTTACAC1601.0061977E-438.2812463
AGATACC1953.2179584E-431.4102576
ATAGACT2352.6139442E-531.2765964
CTATACA2802.453231E-630.6254
TATAGGT2403.0182564E-530.6253
ACACACT2104.967655E-429.1666666
TCTATAC3004.1992553E-628.5833343
GCTATAC1750.00719432828.0000023
CCCAAAG2257.436947E-427.2222211
GCCTTAA1850.00943449726.4864861
TGTAAGC1850.00943449726.4864862
GTGTGTG2400.001084006325.5208349
TATAGTG2400.001084006325.5208345
GTATATA6259.094947E-1225.481
CCAGTAT2450.001222501425.03
TAAACTC2500.001375249724.55
ATAGTGG2550.001543374624.0196086
GTACATT3101.7226803E-423.7096774
Produced by FastQC (version 0.11.2)