FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji7.35200000056f62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji7.35200000056f62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142561
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC600.003770294661.2500048
GTACGCA700.00693961452.59
TATACGG1653.8689905E-851.9696965
GCATAGG953.5781652E-451.5789451
ATACGGT1601.8672727E-645.9375046
TATAGAC1651.2047947E-437.1212122
TACAGGC1701.4361189E-436.029417
GTATAGA2501.010616E-634.3000031
GTGTGCA1450.002865303333.7931029
TATATAG4206.366463E-1132.0833325
ATACAGG2302.2534532E-531.956526
GTGTACA1550.003972357631.6129021
TACACCG1600.00464001830.6250025
CGGTGTT1650.00539348629.696979
TAGGATT1650.00539348629.696974
TAGATTG1700.006240575628.8235285
TACAAGG1700.006240575628.8235282
TACGGTG1750.0071895328.0000027
CCTCTGT1800.00824903827.222223245
TACATTG2859.720691E-525.7894745
Produced by FastQC (version 0.11.2)