FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji6.35200000056f56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji6.35200000056f56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146673
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCT500.001830220773.54
GAGGAGC700.0069400152.58
TAGGCTC700.0069400152.55
TACAGGC1302.953398E-547.1153837
GTATACG1200.001131440440.8333361
TAATGCG1300.001676872837.6923079
TATACAG3352.0008883E-1036.5671655
GATTGTC1450.002865570833.7931027
TCTATAC1953.2151706E-431.4102573
ATACAGG2403.0146046E-530.6250026
GAAGTGT2054.3101827E-429.8780486
CTACAAC2054.3101827E-429.8780483
TATAGGG1650.00539398529.696972
CATAAGT1700.00624115328.8235284
CATATAC2605.2046715E-528.269233
GTACAGA1750.00719019428.0000021
ACAGGCA1750.00719019428.0000028
TATATAC6301.0913936E-1125.2777793
AGAAGTG2951.228956E-424.9152555
ATATACA6853.092282E-1123.2481774