FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji5.35200000056f49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji5.35200000056f49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171670
Sequences flagged as poor quality0
Sequence length251
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCG953.5802272E-451.5789457
ATGGCGT1107.3763187E-444.5454568
ACAGCGT1200.001131980640.8333328
ATACCGT1701.4373416E-436.029416
TATAGGG1400.002413723635.02
GTATAGG1450.002866926133.7931021
GGCCAAT1500.003385024832.6666681
GTATATG3807.7852746E-1032.2368431
TGTACAG1700.006244078728.8235285
AACAGCG2155.7001284E-428.4883737
CTATAAC1800.00825365427.2222233
ATAGGGA1850.00943349226.4864863
ATGTAGC1850.00943349226.4864866
GTGCTAC1850.00943349226.4864861
AGGGAGG1850.00943349226.4864865
TTAGTGT2359.585483E-426.0638314
ATATGGC2859.732046E-525.7894736
TGTATAC3001.3788295E-424.53
TACAAGT2650.001930255623.1132074
GTATTGG3202.1357076E-422.9687521