FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji4.35200000056f3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji4.35200000056f3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125954
Sequences flagged as poor quality0
Sequence length251
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCT500.001829618173.56
TACTCGT550.002670037866.818185
GCGTATA1302.9510027E-547.1153831
CTATAGG1107.368808E-444.5454521
GTACTAG1250.001382200239.21
ATATAAG1852.35887E-433.108111
GCTGTTC1852.35887E-433.108118
CTAATAC1500.00338162632.6666683
TATAGCA1902.758568E-432.2368432
GTATTAG1953.21261E-431.4102571
GTCACAT1600.00463798630.6250021
GACAGTA1650.00539112729.696977
CTATAGA1650.00539112729.696971
TAATAGA3352.9801777E-729.253734
TATTCGT1700.006237852428.8235282
GCGGCGA350.00720793327.99999845-49
CATGCTT1800.0082454527.2222234
GTGTATA2308.4418897E-426.6304341
TTAGACA1850.00942413326.4864864
GGATATA2550.001540853924.0196081
Produced by FastQC (version 0.11.2)