FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji3.35200000056f2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji3.35200000056f2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132303
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAGT700.006938516652.52
GGTATTC1004.6074056E-448.9999967
GTCTAGA1300.001676278837.6923071
TCATAGC1300.001676278837.6923072
TAGGTAT1651.20432545E-437.1212125
GTACTAG1651.20432545E-437.1212121
TATTAGG2653.806599E-836.9811332
TCTAAGT1751.7023309E-435.03
CCTTTAG2251.9379362E-532.6666681
TCTATGG1550.003971332731.6129062
TATTAGC1953.2134805E-431.4102572
GTATTAG2352.6088599E-531.2765961
GTACCAC1650.005392129.696976
ATAGCCT1700.006238973228.8235284
GCACAGA1700.006238973228.8235281
TACTAGC1800.0082469327.222222
CAGAGAT1800.0082469327.222224
ACTGAAT2308.4441504E-426.6304363
GTATTAA2859.715505E-525.7894741
TATTCAG2500.001373366724.55
Produced by FastQC (version 0.11.2)