FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji2.35200000056f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji2.35200000056f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154889
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTG650.00517703156.538468
CTATGCT1200.001131636640.8333364
CCTTATG1250.001383183239.1999972
TACAGGC1300.001677162637.6923079
GGCTATA1300.001677162637.6923071
TTCTAGG1802.0103874E-434.027782
AATAGGG1500.003384007832.6666685
GAGGGCA1550.00397340931.6129026
GAGAGGG2155.697584E-428.488373245
TACTGGA1800.008251199527.2222232
ACCATCC2359.581226E-426.0638318
CTATACC2359.581226E-426.0638313
TACATTG2859.726009E-525.7894745
TTAATAG3101.7212381E-423.7096773
TGCGCAG1050.005932925323.3333328
TTAATAC3802.6236823E-522.5657883
TATGATA4503.9116567E-621.7777774
GTATTAA2850.002944259421.4912281
ACATTGT2850.002944259421.4912286
AAGAGAC2900.003256752621.120695