FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_viji1.35200000056f05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_viji1.35200000056f05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157132
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG407.546584E-491.874999
ATCCGAG500.001830464373.57
CTAGGAC600.003771011361.253
CAGAGCC700.006940926452.54
GTGTGGG1404.5766268E-543.751
ACTGTCC1506.875402E-540.8333368
TACTCAG1250.001383244239.1999975
TCTATCC1957.2792063E-637.6923073
GCATACA1350.002018716436.29631
GTACAGT1802.0105208E-434.0277756
CAGCTCA1802.0105208E-434.0277759
GGTATAA1450.002866189733.7931021
TAATACC2251.9402407E-532.6666684
GTATATG3409.391442E-932.426471
GTATATA4901.2732926E-1130.01
AGAGTCC1650.005395141429.696978
AGTACAG1750.007191732527.9999985
AGAGAAC2257.432934E-427.2222213
CTGTACA1800.00825156127.222224
GAGTATG1800.00825156127.222221
Produced by FastQC (version 0.11.2)