FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_ny238plasmidsmix4.3520000005706e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_ny238plasmidsmix4.3520000005706e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152547
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAATTGTGGAGATGCA11950.7833651268133756No Hit
CAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAGAAGGTAGACTT9300.6096481740053885No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA8560.5611385343533468No Hit
GAATATATAATGAAGGGGGTATACATTAATACTGCCTTGCTTAATGCATC8080.5296728221466172No Hit
CATTAATACTGCCTTGCTTAATGCATCCTGTGCAGCAATGGACGATTTCC7750.5080401450044905No Hit
CTTTATCTGACTCGTTTTCTTCATGTTTTTGGTTCTTGGAATCTTCTCCT7570.4962405029269668No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA5040.3303899781706622No Hit
ATATATAATGAAGGGGGTATACATTAATACTGCCTTGCTTAATGCATCCT4890.3205569431060591No Hit
TCATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAA4740.31072390804145605No Hit
ATATGATAGTGATGAACCTGAATTAAGGTCACTTTCAAGCTGGATCCAGA4560.2989242659639324No Hit
GTCCAAATTCCTGCTGATGGATGCTTTAAAATTAAGCATTGAGGACCCAA4430.2904023022412765No Hit
TCTTAGTAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTTGT4360.2858135525444617No Hit
ATATAATGAAGGGGGTATACATTAATACTGCCTTGCTTAATGCATCCTGT3680.24123712691826124No Hit
GCTTAATGCATCCTGTGCAGCAATGGACGATTTCCAACTAATTCCCATGA3420.22419319947294933No Hit
ATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATT3390.2222265924600287No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTATTCTCGAAAAA3180.20846034336958444No Hit
GGATAGAGCTCGATGAGATTGGAGAAGATGTGGCTCCAATTGAACACATT2950.19338302293719312No Hit
GTGCAGCAATGGACGATTTCCAACTAATTCCCATGATAAGCAAATGTAGA2940.19272748726621958No Hit
CTATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTAC2900.19010534458232545No Hit
GCATTGAGGACCCAAGTCACGAAGGAGAGGGAATACCACTATATGATGCG2890.1894498089113519No Hit
TCTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCAAG2850.18682766622745778No Hit
ATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACCCT2850.18682766622745778No Hit
ATGCATCCTGTGCAGCAATGGACGATTTCCAACTAATTCCCATGATAAGC2770.1815833808596695No Hit
GAGTTCAACAAGGCATGCGAGCTGACCGATTCAATCTGGATAGAGCTCGA2680.17568355982090764No Hit
TTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTGCTGTCATGG2490.16322838207241047No Hit
ATTGAATACCGACTTGGCTAATAAAGTCCTGCAGACCTTCCCAATGGAAC2450.16060623938851634No Hit
TTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATTC2400.15732856103364865No Hit
GTAGAGATGTAAGCGATTTGAAGCAATATGATAGTGATGAACCTGAATTA2340.15339534700780744No Hit
GTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGACACCTCTG2310.15142873999488682No Hit
TACAAGAACATGGGCCTTGACACCTGGCCTATGGCACTTCTTAGTAGCAT2270.1488065973109927No Hit
CTGTAGAGATGTAAGCGATTTGAAGCAATATGATAGTGATGAACCTGAAT2270.1488065973109927No Hit
CTCAAGAACACAGTACTTCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAG2190.1435623119432044No Hit
CTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACC2180.14290677627223086No Hit
CATACAAGAACATGGGCCTTGACACCTGGCCTATGGCACTTCTTAGTAGC2170.1422512406012573No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG2150.14094016925931024No Hit
CTCCTATCTCAAGAACACAGTACTTCTCCCATTTGTGTGGCTCAAGTCTT2090.13700695523346904No Hit
ATCCAGATTGAATCGGTCAGCTCGCATGCCTTGTTGAACTCATTCTGGAT2070.13569588389152196No Hit
CTCCTTGACAGAGGACTCGGCTTCAATCATACTCTCGATTTGTTGGAGTG1990.13045159852373367No Hit
GCATTAAGCAAGGCAGTATTAATGTATACCCCCTTCATTATATATTCTGT1990.13045159852373367No Hit
ATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATTCCT1990.13045159852373367No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT1910.12520731315594538No Hit
CCTTAAGTGAGATCTTCCTTTTATGATGAAACCATATAAATTGGTCTTTC1900.12455177748497184No Hit
GGTGAGAACATGGCACCAGAGAAGGTAGACTTTGACGACTGTAGAGATGT1840.12061856345913062No Hit
CTCCAATGGGCCATGTTTCTGATTTATTCTCGAAAAACTCTTTGGTCATG1830.1199630277881571No Hit
GGGGGACTCTCCAATGGGCCATGTTTCTGATTTATTCTCGAAAAACTCTT1800.11799642077523649No Hit
GTGTATGAGAACATTCTTTGGATGGAAAGAACCCTATGTTGTTAAACCAC1770.11602981376231587No Hit
GTACTTCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAGTGAGAGAAAACT1750.11471874242036881No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG1750.11471874242036881No Hit
GGCTAATAAAGTCCTGCAGACCTTCCCAATGGAACCTTCTTCCACTCCTT1720.1127521354074482No Hit
ATTTATATGGTTTCATCATAAAAGGAAGATCTCACTTAAGGAATGACACC1690.1107855283945276No Hit
GCATACAGGCTATTGAATACCGACTTGGCTAATAAAGTCCTGCAGACCTT1680.11012999272355406No Hit
GAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGG1660.10881892138160697No Hit
GATGGATGCTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGG1660.10881892138160697No Hit
TCAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAGAAGGTAGACT1650.10816338571063346No Hit
TTGTTGGAGTGACTGAAGGAGGCAACGCCTCATCTCCATTCCCCATTTCA1650.10816338571063346No Hit
CTTCTTAGTAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTT1630.10685231436868636No Hit
GGAGAGGGAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTT1610.1055412430267393No Hit
GACTGAAGGAGGCAACGCCTCATCTCCATTCCCCATTTCATTTTAATCTT1600.10488570735576576No Hit
TCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAGTGAGAGAAAACTCCATG1590.10423017168479222No Hit
GTGTTCAATTGGAGCCACATCTTCTCCAATCTCATCGAGCTCTATCCAGA1540.10095249332992455No Hit
GCCTTGCTTAATGCATCCTGTGCAGCAATGGACGATTTCCAACTAATTCC1530.10029695765895101No Hit
GGCTATTGAATACCGACTTGGCTAATAAAGTCCTGCAGACCTTCCCAATG1530.10029695765895101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACC100.00147495245.00002245
TCCTGCT800.0153.1259
ACTATCC302.4032594E-4122.5245
TGCTAAC551.7849925E-7111.363642
AGCAGTT3450.0102.971013
CTAACCA602.9981493E-7102.0833364
GTCCAAA1250.098.01
GCAGTTT3650.097.3287664
GAAGCAG3850.092.272721
GACTATG407.546155E-491.875012
CAAATTC1750.084.000014
AAGCAGT4450.079.831462
CAGTTTT4550.078.076935
TATCTGA3150.070.000014
TTCCTGC1800.068.055568
CGGTTGT550.00267111966.818184245
TTTATCT3300.066.8181842
TAAGATA954.609812E-664.473694
GGACCCA1950.062.8205158
TTTCTTG6000.059.2083329
Produced by FastQC (version 0.11.2)