FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_ny238plasmidsmix3.35200000057052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_ny238plasmidsmix3.35200000057052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173115
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATCTGACTCGTTTTCTTCATGTTTTTGGTTCTTGGAATCTTCTCCT21361.2338618837189153No Hit
GCCTTGTTGAACTCATTCTGGATCCAGCTTGAAAGTGACCTTAATTCAGG7400.42746151402247057No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA6970.40262253415359733No Hit
CTATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTAC6970.40262253415359733No Hit
TCTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCAAG6770.39106952026109815No Hit
CACAGGGACAATGCGCAGGCTCGCCGACCAAAGTCTCCCGCCGAACTTCT5040.29113595009098No Hit
ATATAGTGGTATTCCCTCTCCTTCGTGACTTGGGTCCTCAATGCTTAATT4960.2865147445339803No Hit
TTGTTGAACTCATTCTGGATCCAGCTTGAAAGTGACCTTAATTCAGGTTC4090.23625913410160876No Hit
GTTGAACTCATTCTGGATCCAGCTTGAAAGTGACCTTAATTCAGGTTCAT4020.23221557923923403No Hit
ATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACCCT3640.21026485284348553No Hit
ATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTACCT3460.19986714034023628No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG3390.19582358547786155No Hit
CATCTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCA3090.17849406463911274No Hit
TTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTGCTGTCATGG3060.17676111255523785No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT3020.174450509776738No Hit
ATGTGGATGGATTCGAACCGAACGGCTACATTGAGGGCAAGCTTTCTCAA2820.1628974958842388No Hit
GTTCTCAGCGGTCCAAATTCCTGCTGATGGATGCTTTAAAATTAAGCATT2760.15943159171648905No Hit
CTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACC2740.15827629032723914No Hit
GATGGATGCTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGG2710.15654333824336425No Hit
GAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGG2700.1559656875487393No Hit
TCCTTGTTCTCAGCGGTCCAAATTCCTGCTGATGGATGCTTTAAAATTAA2630.15192213268636456No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG2490.1438350229616151No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2430.14036911879386535No Hit
GCTTGAAAGTGACCTTAATTCAGGTTCATCACTATCATATTGCTTCAAAT2410.13921381740461541No Hit
GTGTATGAGAACATTCTTTGGATGGAAAGAACCCTATGTTGTTAAACCAC2330.13459261184761573No Hit
CAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAGAAGGTAGACTT2270.131126707679866No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2170.12535020073361638No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC2150.12419489934436646No Hit
GGAGAGGGAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTT2140.12361724864974151No Hit
GTTTAACAACATAGGGTTCTTTCCATCCAAAGAATGTTCTCATACACTTG2120.12246194726049159No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG2120.12246194726049159No Hit
CTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGGGAATACCA2080.12015134448199175No Hit
CTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCAAGT2060.11899604309274182No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT1990.11495248823036709No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC1970.11379718684111717No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1940.1120642347572423No Hit
ACCCTATGTTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTGC1940.1120642347572423No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG1940.1120642347572423No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA1930.11148658406261733No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC1920.11090893336799237No Hit
GCTACATTGAGGGCAAGCTTTCTCAAATGTCCAAAGAAGTAAATGCTAAA1870.10802067989486756No Hit
GTCCTGCAGTTCTGCCAGTACTTGCTTCCATGACAGCAGATAATTTGGAT1840.10628772781099269No Hit
GTCCAAAGAAGTAAATGCTAAAATTGAACCTTTTCTGAAAACAACACCAA1760.10166652225399302No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC1750.10108887155936806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTA100.001475122244.999982
ACGTCGT100.001475122244.99998245
CTAAAGG301.1750635E-6163.33333245
CTAGCCC150.0049633393163.33333245
CACGCAA302.4038149E-4122.5245
TTGAACT2800.083.1257
CAGGGAC2100.075.8333363
CAATGCG2100.075.8333369
GGGACAA2150.074.069775
TATCTGA8000.071.968744
TTTATCT7850.071.783442
GTTGAAC3850.060.4545446
GCCTTGT3750.058.81
GCGATGA1501.8277206E-857.1666689
AGTGAAG650.005178057656.5384642
GGTTGAC650.005178057656.5384648
GTGAAGC650.005178057656.5384643
TAGCGAT1552.3672328E-855.322587
CTTGTTG4050.054.4444473
AGCGATG1603.0408046E-853.5937468
Produced by FastQC (version 0.11.2)