FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_ny238plasmidsmix2.35200000057045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_ny238plasmidsmix2.35200000057045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178589
Sequences flagged as poor quality0
Sequence length251
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG4120.23069729938574043No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT3940.22061829116014983No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA3440.19262104608906483No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3420.19150115628622144No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG3020.16910336022935343No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC2970.16630363572224494No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT2860.16014424180660622No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT2810.15734451729949772No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG2760.15454479279238925No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT2660.14894534377817223No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2600.14558567436964204No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC2390.13382683143978633No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG2160.12094809870708723No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT2160.12094809870708723No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC2050.11478870479144854No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT1920.10750942107296642No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1900.10638953127012302No Hit
CCATAAGAGAAGAGGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAGGA1860.10414975166443623No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT1840.10302986186159283No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT1840.10302986186159283No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT1830.10246991696017112No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1820.10190997205874942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCC150.0049634697163.33333245
TATGGTT603.0002593E-7102.0833365
CAGAACC603.684737E-581.6666649
TGGGCCC801.5318199E-461.2500046
AGGATCA600.00377185561.259
TGAGGCC700.00694247352.59
TTAGACA953.580617E-451.5789534
GGTTGCT1459.4885945E-750.689658
TATTACT750.00911912649.0000044
TCCCGTG1252.3443306E-549.07
TAGCGAT1756.1620085E-848.9999967
TAATAGC1807.6917786E-847.6388854
ATAGCGA1807.6917786E-847.6388856
AATAGCG1807.6917786E-847.6388855
TCTTAGA1302.9560031E-547.1153832
GACTATG1302.9560031E-547.1153832
CTTAGAC1353.6943115E-545.370373
AGCGATG1901.1769043E-745.131588
TACTGAT3051.8189894E-1244.180337
GTAATAG1951.4435136E-743.974363
Produced by FastQC (version 0.11.2)