FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_ny238plasmidsmix1.35200000057038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_ny238plasmidsmix1.35200000057038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188595
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC3520.18664333624963544No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3270.17338741748190567No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT3040.16119197221559425No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT3010.1596012619634667No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2870.152177947453538No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT2680.14210344919006335No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2510.13308942442800709No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG2140.11347066465176703No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT2040.1081682971446751No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC2030.1076380603939659No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC2000.10604735014183833No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1920.10180545613616479No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC1920.10180545613616479No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAC450.001205262381.666677
TATAAGT500.001831035573.52
TGCTAAC550.002672101466.8181842
TTAGACA650.005178774756.538464
GTATAAG1201.8418496E-551.0416681
CTAACCA750.00911991649.04
TAAGGGT750.00911991649.04
ATAGTGG750.00911991649.06
TGATGCG1107.378175E-444.5454569
TTATATG1200.001132264540.8333324
GTATATA4800.040.8333321
TATATAT3650.040.273972
TAAAGTA2502.2868335E-839.24
GTATATG2502.2868335E-839.21
CTGTACT1350.002019740936.29634
ATTATAT1350.002019740936.29633
ATTATCT2051.0268144E-535.8536576
TTATTAT2101.2111705E-535.0000044
ATTACTC1450.002867638633.7931023
TGAGTAA4003.6379788E-1133.68758
Produced by FastQC (version 0.11.2)