FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-7c.352000000570e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-7c.352000000570e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138141
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT3080.22296059822934539No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2570.18604179787318753No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA2550.1845940017807892No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2480.179526715457395No Hit
CCATATACACTGGTCCTTGAAACAGTGTTTCTAGGGAATCTGTAATTTGC2410.17445942913400075No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2370.17156383694920407No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2360.17083993890300492No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2310.16722044867200903No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2270.16432485648721235No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2250.16287706039481398No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2130.1541902838404239No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2080.15057079360942804No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC1850.13392113854684706No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1740.12595826003865615No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1730.12523436199245697No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1670.12089097371526195No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1660.12016707566906276No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1640.11871927957666444No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1590.11509978934566856No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1590.11509978934566856No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1570.1136519932532702No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1550.11220419716087186No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1540.11148029911467269No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1500.10858470692987598No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1480.10713691083747766No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1470.10641301279127847No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1440.10424131865268095No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1420.10279352256028261No Hit
GTACAAGACACTGTTTCAAGGACCAGTGTATAAAGATTTAGAGATTGATG1410.10206962451408344No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1390.10062182842168509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA100.0014747997245.000022
GTACCGA100.0014747997245.000026
CGGGTAC150.0049622594163.333333
GGTACCG150.0049622594163.333335
CCGAGCT150.0049622594163.333339
TACCGAG150.0049622594163.333337
TCGAGGG450.00120457581.66667245
CGTCTAT902.7395497E-454.4444471
GGGAGTG2056.548362E-1153.7804877
CACTAGG1252.3415452E-549.01
TATAGGG2302.0372681E-1047.934783
ACTAGGA1302.952495E-547.1153832
GATATAT1601.8668452E-645.9375042
GGAAGAT1455.6259763E-542.241386
CTATATC1200.001131211240.8333324
ACCTGTG1200.001131211240.8333327
ATGTAAG1200.001131211240.8333326
TCATGGC1200.001131211240.8333329
GGTTATA1855.066988E-639.72973245
ACTGCAA1250.001382663839.1999976
Produced by FastQC (version 0.11.2)