FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-6f.352000000570d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-6f.352000000570d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139272
Sequences flagged as poor quality0
Sequence length251
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT3020.21684186340398645No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA2800.2010454362685967No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2680.19242920328565682No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2520.18094089264173704No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2480.17806881498075708No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2460.1766327761502671No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2440.1751967373197771No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2390.17160664024355218No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2320.16658050433683727No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2110.1515020966166925No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1970.14144982480326268No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC1960.1407318053880177No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1860.1335516112355678No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC1820.13067953357458784No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1750.12565339766787295No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1710.12278132000689299No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1660.11919122293066804No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1630.11703716468493307No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1600.11488310643919812No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1530.1098569705324832No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1510.10842093170199323No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1500.10770291228674825No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1490.10698489287150324No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1470.10554885404101327No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1460.10483083462576828No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT1450.1041128152105233No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1440.1033947957952783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG150.0049623027163.333333
AGACGTT150.0049623027163.333335
ATATGAG600.003770111661.255
AGGAGGC700.006939278452.54
GAGTAAA700.006939278452.59
GGGAGTG1902.0154403E-951.5789457
CCTAAGT953.577877E-451.5789454
CTCCTAA953.577877E-451.5789452
AGTACGC953.577877E-451.5789458
CATATCA1201.83934E-551.0416682
TATAGAC1201.83934E-551.0416682
GACGCGG150.00913016149.000004240-244
CTTACGT150.00913016149.000004220-224
AAGTACG1004.6082446E-449.07
TTAGGAG750.00911494149.02
TAGAGAG1004.6082446E-449.05
AAGAGGT750.00911494149.07
AGGCGCT750.00911494149.07
ATATCAC1252.3416458E-549.03
ACCTAAA750.00911494149.01
Produced by FastQC (version 0.11.2)