FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-6c.352000000570cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-6c.352000000570cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152142
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA3310.2175599111356496No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3300.21690263043735455No Hit
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT3020.1984987708850942No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2890.189954121807259No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2860.18798227971237397No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2770.18206675342771886No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2660.1748366657464737No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2550.16760657806522855No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2380.15643280619421332No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2310.15183184130614819No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2290.15051727990955818No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2180.14328719222831302No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2070.13605710454706788No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1940.12751245546923268No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1840.12093964848628257No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1820.11962508708969252No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1780.11699596429651246No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1750.11502412220162743No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1720.11305228010674238No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1660.10910859591697229No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1650.10845131521867728No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1620.10647947312379225No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1600.10516491172720221No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1580.10385035033061218No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1580.10385035033061218No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1570.10319306963231718No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1530.10056394683913712No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC100.0014749463245.000023
CCGGGTA100.0014749463245.000022
GTACCGA100.0014749463245.000026
GGTACCG100.0014749463245.000025
GGGTACC100.0014749463245.000024
CCGAGCT100.0014749463245.000029
GTCCGAA100.0014749463245.00002245
CAGAGAG150.004962752163.333332
GACGTTG404.9162754E-6122.500016
CGTTGTG404.9162754E-6122.500018
AGACGTT404.9162754E-6122.500015
ATAGACG603.682979E-581.6666643
GTTGTGG751.1125786E-465.3333369
GGCAGAG1006.2491654E-661.257
AGGCAGA1006.2491654E-661.256
CCTCTAC600.00377078261.249996245
GTACTCC650.005176854356.538469
TTAGACG750.00911654849.06
GGGAGTG2251.6370905E-1049.07
TATAGGG2251.6370905E-1049.03
Produced by FastQC (version 0.11.2)