FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-5d.352000000570be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-5d.352000000570be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108896
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2320.21304731119600354No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2160.19835439318248602No Hit
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT2100.19284454892741698No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT1980.18182486041727888No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA1900.17447840141052012No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA1790.16437702027622686No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC1740.15978548339700263No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT1680.1542756391419336No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG1580.14509256538348517No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC1520.1395827211284161No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC1490.13682779900088157No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT1470.1349911842491919No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1460.13407287687334707No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1390.12764472524243314No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1360.12488980311489861No Hit
GTACAAGGATATAAAATCATATGGTTTAGGACCTAGGTACGGTGGGATTT1310.12029826623567441No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1300.11937995885982958No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1280.11754334410813988No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1200.11019688510138113No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1200.11019688510138113No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1200.11019688510138113No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1200.11019688510138113No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1160.10652365559800177No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1140.10468704084631208No Hit
TTTAAGGACATGGTGTGAAGACTCGCATGTGCTTGGTTGTGAGTCCTCCG1130.10376873347046724No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1100.10101381134293269No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1090.10009550396708786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAG150.004960823163.33333245
GGTACAA150.004960823163.33333245
ATAGACG450.001203885381.6666645
GATATAG450.001203885381.6666642
GCGGTGC500.001828948273.499999
AGACGTT500.001828948273.499997
GACGTTG550.002669060866.8181848
GTAGGCG550.002669060866.8181845
TAGGCGG550.002669060866.8181846
GGCGGTG600.003767900461.258
TGTAGGC600.003767900461.254
TACTGGC1006.2372565E-661.2499967
ACTGGCC1058.329449E-658.3333328
ATGTCGG150.00912909949.000004230-234
TAAAGGA750.0091096349.04
GTAAAGG1004.6037772E-448.9999963
AAGGACA1302.9483374E-547.1153834
CTATACA2702.0008883E-1145.370374
TAAGGAC1455.6180877E-542.241383
GTCTATA1506.8614514E-540.8333321
Produced by FastQC (version 0.11.2)