FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-5c.352000000570a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-5c.352000000570a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145785
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2820.1934355386356621No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2810.1927495970092945No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA2780.19069177213019173No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2670.18314641424014816No Hit
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT2560.17560105635010462No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2430.16668381520732586No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2120.14541962478993037No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2100.14404774153719518No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2070.1419899166580924No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT2070.1419899166580924No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2050.1406180334053572No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2030.139246150152622No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC1980.13581644202078402No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC1960.13444455876804884No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1870.12827108413074045No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1860.1275851425043729No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1760.12072572624069691No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1750.12003978461432932No Hit
GTACAAGGATATAAAATCATATGGTTTAGGACCTAGGTACGGTGGGATTT1620.11112254347155058No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1610.11043660184518297No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1480.10151936070240423No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1470.10083341907603663No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1460.10014747744966904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTC150.0049625393163.33334245
AGACGTT302.4030427E-4122.500015
GACGTTG407.5454795E-491.875016
ATAGACG407.5454795E-491.875013
CGTTGTG500.001830197973.58
ACATCTC550.002670881866.818184245
GATATAT2350.062.5531922
TAGACGT852.0659204E-457.6470574
TATAGGG2253.6379788E-1254.4444473
CGTGACT150.00913033149.000004240-244
ATATGTT3100.047.4193535
GGGAGTG2601.2732926E-1147.1153837
CTGTCGG1159.176505E-442.6086969
GAGGTGT1455.6275163E-542.241386
GCTGTGC2102.5790177E-740.8333328
CTGTGCG2153.1019772E-739.8837249
TTATAGG3108.731149E-1139.516132
GAGTGGC1250.001382915339.27
CAGGCTG2254.4305307E-738.1111155
CCATGCA1300.001676838837.6923079
Produced by FastQC (version 0.11.2)