FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-5b.35200000057095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-5b.35200000057095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158511
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT3230.20377134709893951No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3180.20061699188068968No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA3090.19493915248783997No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2960.1867378289203904No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2870.18105998952754068No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2800.17664389222199092No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2710.17096605282914118No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2680.1690734396981913No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2520.15897950299979183No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2470.15582514778154197No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2360.14888556630139232No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2340.1476238242140924No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT2330.14699295317044242No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2180.13752988751569292No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2170.13689901647204294No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1880.11860375620619389No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG1870.11797288516254394No Hit
GTACAAGGATATAAAATCATATGGTTTAGGACCTAGGTACGGTGGGATTT1820.11481852994429409No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1810.11418765890064413No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1760.11103330368239428No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1760.11103330368239428No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1710.10787894846414445No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1680.10598633533319454No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1670.10535546428954458No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1590.10030849594034484No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1590.10030849594034484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAC150.004962948163.33333245
ACAAACG251.16284784E-4147.0245
CTATATC1151.9119216E-763.913043
GTCTATA1357.947165E-963.518521
CATGGCC1202.566303E-761.2499969
CTTCATG801.5312918E-461.2499962
CATGTGG801.5312918E-461.2499965
ATATCAT1253.40302E-758.8000035
TCATGGC1253.40302E-758.8000038
ACCTAAA650.005177255356.538461
TCTATAT1552.3655957E-855.322582
ATAGGTC902.740789E-454.4444473
CGTCTAT902.740789E-454.4444471
CTGTGCG2353.6379788E-1252.1276639
GCTGTGC2405.456968E-1251.0416648
GAGTAAA1252.3431268E-549.09
ATATGAG1252.3431268E-549.05
CTATGCA150.00913062748.999996220-224
CCGTCAC150.00913062748.999996210-214
CGTCCGG150.00913062748.999996235-239
Produced by FastQC (version 0.11.2)