FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-3e.35200000057088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-3e.35200000057088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152561
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3120.20450836059019017No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2820.18484409514882572No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2770.181566717575265No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2700.17697838897227994No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2630.1723900603692949No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2440.1599360255897641No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2380.1560031725014912No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2300.15075936838379403No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2260.14813746632494545No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2200.14420461323667255No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1970.12912867639829315No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1900.12454034779530812No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1880.1232293967658838No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1860.1219184457364595No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1710.11208631301577729No Hit
TACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGG1580.10356513132451937No Hit
TATATATACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCA1560.10225418029509509No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1540.10094322926567079No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG150.0049627647163.33334245
GATATAT1401.3460522E-1070.02
GACGTTG650.00517688456.5384566
ATAGACG650.00517688456.5384563
AGACGTT700.00694054352.55
GTATATA3100.051.3709641
GATACAG150.00913049349.000004225-229
AAGACAG150.00913049349.000004210-214
TAGTTCA150.00913049349.000004225-229
CCTGCGG150.00913049349.000004185-189
GCCACTA150.00913049349.000004240-244
GCCTACG204.6213416E-449.0225-229
GATTAGC750.00911659949.0245
AGTAACG204.6213416E-449.0235-239
TAGAGTG1807.681956E-847.6388855
GAGTGGC1807.681956E-847.6388857
GTGCTAG1807.681956E-847.6388851
GGGAGTG2352.5283953E-1046.9148947
TATCCCG1652.3089397E-644.5454565
TATAGGG2504.638423E-1044.13
Produced by FastQC (version 0.11.2)