FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_hrv14-3d.3520000005707b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_hrv14-3d.3520000005707b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137499
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2790.2029105666223027No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2530.18400133819155048No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2460.17891039207557874No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2350.1709103338933374No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2280.16581938777736566No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2250.16363755372766348No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2180.15854660761169173No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2140.15563749554542214No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2120.15418293951228737No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1990.14472832529691124No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC1930.1403646571975069No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1820.13236459901526557No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1670.12145542876675468No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1650.1200008727336199No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1650.1200008727336199No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1650.1200008727336199No Hit
TATATATACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCA1610.11709176066735032No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1590.11563720463421553No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1540.11200081455137856No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1480.1076371464519742No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG1470.10690986843540681No Hit
TCACCAGGCTGTGCGTGTGTGGGTATCACACAGACACGATCATGTATGCC1410.10254620033600245No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1400.10181892231943504No Hit
GTACAAGGATATAAAATCATATGGCGCTCAGGTTTCTACACAGAAAAGTG1390.10109164430286766No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAC150.004962234163.33333245
CGTAGAG150.004962234163.33333245
GCTTAAC655.4750453E-575.384612
ACCGCTC852.3804314E-672.058828
CTGATAC852.3804314E-672.058823
TTAACTA707.903848E-570.04
TCCGACC1101.4042598E-766.8181848
TTCCGAC1151.9100298E-763.913047
TGTTCCG1151.9100298E-763.913045
TAGGGGG600.003770008261.25245
GTTCCGA1202.5637746E-761.256
CCGACCC1202.5637746E-761.259
CCGCTCG1058.3410705E-658.3333329
CTATGCG852.0655044E-457.6470578
GGCTTAA852.0655044E-457.6470571
TCTCGGG1355.785114E-754.444447245
ATACTCT750.00911469349.04
CTTAGGG1551.5000969E-647.419357245
TATCAAG1302.9524217E-547.1153839
ACTATGC1055.8612577E-446.6666647
Produced by FastQC (version 0.11.2)