FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_98.35200000056e8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_98.35200000056e8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184435
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7060.38279068506519914No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5390.2922438799577087No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT5150.279231165451243No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3600.1951907175969854No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2840.1539837883265107No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2650.14368205600889203No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2400.13012714506465692No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2360.1279583593135793No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2320.1257895735625017No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2210.11982541274703826No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2110.11440344836934423No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2110.11440344836934423No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2100.11386125193157481No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2040.1106080733049584No Hit
ATTCTAGAGCTACTGAATACATAATGAAGGGGGTATACATTAACACTGCC1990.10789709111611136No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1970.10681269824057256No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1920.10410171605172555No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1920.10410171605172555No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT100.0014751999244.999981
TACGCCC100.0014751999244.99998245
CAAGCTA254.7392132E-7196.04
GCAGGGG1300.0169.615399
CTAAATT301.1752709E-6163.333338
GCAGGGT1100.0144.772749
GCTAAAT352.531613E-6140.000027
AAGCTAA352.531613E-6140.000025
GCAAAAG7850.0110.796183
CGAAAGC1500.0106.166664
AAGCAGG9700.0106.082477
AGCAGGT4450.0104.606748
AGCAAAA9050.097.4585652
CAAAAGC9850.089.543154
TCCAACT852.356137E-886.470592
GAGCAAA10300.085.6310651
AGCAGGG4600.082.554358
CTCGCCG450.001205219981.66667245
GTATTAA603.685058E-581.6666641
AGGGTGT603.685058E-581.6666646
Produced by FastQC (version 0.11.2)