FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_944_nw8.35200000057001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_944_nw8.35200000057001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213052
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA6620.31072226498695155No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG5540.26003041510992625No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG4290.20135929256707283No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA4240.1990124476653587No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4010.18821696111747366No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC3650.1713196778251319No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC3170.1487899667686762No Hit
GACTTGAAGATGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG3120.14644312186696207No Hit
GAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATGATCTC2800.1314233144959916No Hit
GGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCCTGCAG2660.12485214877119202No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC2570.12062782794810657No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2550.11968908998742092No Hit
GAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGC2520.11828098304639244No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2340.10983234140022154No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT2270.10654675853782175No Hit
CGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCG2160.10138369975405065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA10500.0145.833331
CAAAAGC12550.0121.035864
GAGCGAA3250.0120.615391
AGCGAAA3600.0108.888892
GCAAAAG14700.0104.1666643
CGAAAGC3800.0103.157894
AGCAAAA15550.098.472672
AAAAGCA15650.097.06075
AAAGCAG20050.095.311726
GCAGGGT5100.086.470599
GCGAAAG4600.085.217393
AAGCAGG24800.077.55047
CGTCAGG2300.069.239138
CCGTCAG2450.065.000017
CATCCCG2650.060.0943373
ATCCCGT2750.057.9090924
GGATATA650.005179695356.538461
GAAAGCA7150.056.538465
GGACAGT3600.054.4444476
GCAGGTA2056.548362E-1153.7804879
Produced by FastQC (version 0.11.2)