FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_930_ulb.35200000056fcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_930_ulb.35200000056fcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189507
Sequences flagged as poor quality0
Sequence length251
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7980.42109262454685054No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5830.30764035101605747No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA5370.2833668413303994No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC5250.27703462141240165No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG5050.26648092154907205No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4450.2348198219590833No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4380.23112602700691795No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4010.21160168225975823No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA3990.21054631227342527No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3270.17255299276543876No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3240.1709699377859393No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG3020.15936086793627677No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2890.15250096302511254No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2810.1482794830797807No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2730.14405800313444886No Hit
GTACTATGTTCTTTCCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2510.1324489332847863No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT2450.12928282332578744No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2360.12453365838728914No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2340.12347828840095618No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT2270.1197844934487908No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2230.1176737534761249No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2160.11397995852395955No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG2130.11239690354446011No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2120.11186921855129363No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2080.1097584785786277No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1980.1044816286469629No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT1960.10342625866062996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.0189.677411
AGCAAAA12900.0135.794592
GCAGGTA2000.0134.759
GAGCAAA13650.0129.230771
GAGGTTA302.4041711E-4122.56
AAAGCAG17000.0121.058826
AGCGAAA3000.0118.4166641
CAAAAGC14900.0118.389274
AAAAGCA14950.0117.993315
GCGAAAG3200.0111.015632
GCAAAAG15900.0110.9434053
GCAGGGG3900.0109.935899
CGAAAGC3350.0106.044783
AAGCAGG19900.0105.263827
AGCAGGT4350.090.1149448
GCAGGTT1300.084.807699
CAAGGTA603.685322E-581.6666644
AGCAGGG14000.081.3758
GTAGAAA2800.078.752
GGTGTTT3000.077.5833368
Produced by FastQC (version 0.11.2)