FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_930_lle.35200000056fa6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_930_lle.35200000056fa6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180576
Sequences flagged as poor quality0
Sequence length251
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA35351.9576244905192275No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT17190.9519537480063796No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC16520.9148502569555201No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG14420.798555732766259No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11070.6130382775119617No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10540.5836877547403864No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA10030.5554447988658515No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA9710.5377237285132022No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA9110.5044967216019848No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA8860.49065213538897745No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC7460.41312245259613684No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA7370.4081384015594542No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG7080.3920786815523658No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT6490.3594054580896686No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA5990.3317162856636541No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG5830.3228557504873294No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5690.31510278220804533No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG5480.3034733297891193No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5380.2979354953039164No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5330.2951665780613149No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT5300.29350522771575405No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG4990.27633794081162505No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG4640.2569555201134149No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC4630.2564017366648946No Hit
GTTCATTGATGCTTAATGCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTC4600.2547403863193337No Hit
ATGCTGGGCCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGC4580.2536328194222931No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC4460.24698741804004962No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4380.2425571504518873No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4030.22317472975367714No Hit
ATGCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT3990.22095959595959594No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT3950.21874446216551482No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA3790.20988392698919012No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3650.2021309587099061No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT3640.2015771752613858No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT3350.18551745525429736No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT3320.1838561049087365No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3230.17887205387205388No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3140.17388800283537126No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC3140.17388800283537126No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3130.17333421938685098No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3030.16779638490164805No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3030.16779638490164805No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC2950.16336611731348574No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2890.160043416622364No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT2870.15893584972532343No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC2840.15727449937976254No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC2840.15727449937976254No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2830.15672071593124226No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2820.15616693248272195No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2730.15118288144603934No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2720.15062909799751906No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC2640.14619883040935672No Hit
CATTAGCAAGATTGCTCAGTTCATTGATGCTTAATGCATTCTGAGTGCGG2640.14619883040935672No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC2640.14619883040935672No Hit
CCATGGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA2570.14232234626971468No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA2490.13789207868155237No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA2480.13733829523303206No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCAAGATTGCTCAGTTCAT2470.1367845117845118No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2380.13180046074782917No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT2370.1312466772993089No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC2310.12792397660818713No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2260.12515505936558569No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2220.12293992557150452No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT2210.12238614212298424No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA2150.11906344143186248No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG2130.1179558745348219No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2110.11684830763778133No Hit
TCAATGAACTGAGCAATCTTGCTAATGTGCTAATTGGGCAAGGGGACGTA2110.11684830763778133No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT2040.11297182349813929No Hit
GAATGCATTAAGCATCAATGAACTGAGCAATCTTGCTAATGTGCTAATTG2000.11075668970405812No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC1990.11020290625553784No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1960.10854155590997697No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG1950.10798777246145666No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT1940.10743398901293638No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG1930.10688020556441609No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCAAGATTGCTC1920.10632642211589581No Hit
AATTAGCACATTAGCAAGATTGCTCAGTTCATTGATGCTTAATGCATTCT1910.1057726386673755No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG1890.10466507177033493No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG1850.10244993797625378No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1840.10189615452773347No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG1830.10134237107921318No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT1830.10134237107921318No Hit
TAGCAAGATTGCTCAGTTCATTGATGCTTAATGCATTCTGAGTGCGGGGT1830.10134237107921318No Hit
CCATGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAG1810.10023480418217261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCGG100.0014751744244.99998245
GAGCGAA2700.0222.31481
GAGCAAA9950.0217.914581
GTTCGGT204.77967E-5183.749987
GGTTCGG204.77967E-5183.749986
GCAGGTA3350.0164.552239
CCCACTA150.004963516163.333334
GGTTATT301.1752036E-6163.333331
TTATTAG301.1752036E-6163.333333
AGCAAAA14100.0154.645392
AAGCAGG19250.0145.727287
AGCGAAA4200.0142.916662
AGCAGGC3800.0141.84218
AGCAGGT12800.0136.855458
GCGAAAG4550.0131.923083
CGAAAGC4850.0123.7628864
GCAGGTC9750.0123.12829
GCAGGGT4200.0116.6666649
AAAGCAG24700.0113.076936
CAAAAGC19400.0113.028354
Produced by FastQC (version 0.11.2)