FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_82.35200000056ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_82.35200000056ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228544
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10110.4423655838700644No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5840.25553066367964156No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5820.25465555866704004No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG5380.23540324838980678No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA4100.17939652758331No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA3960.1732707924950994No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT3940.1723956874824979No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3890.17020792495099413No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3850.1684577149257911No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3790.16583239988798654No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3170.13870414449733967No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT3090.13520372444693365No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2980.1303906468776253No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2960.12951554186502381No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2830.12382735928311396No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2670.11682651918230187No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2620.11463875665079809No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2590.11332609913189583No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2590.11332609913189583No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2490.10895057406888825No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGCGTGCTGTGGCAC2450.10720036404368524No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2440.1067628115373845No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2430.10632525903108372No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2380.10413749649957994No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT2340.10238728647437693No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2330.10194973396807616No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2290.10019952394287315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTACC100.00147543245.00002245
ACGTCAA204.7813228E-5183.75002245
GAGAGAT150.0049643735163.333335
GCAGGGT3050.0148.606559
GCAGGGG1250.0147.09
GCTCCCC505.8935257E-10147.07
GCTAAAT455.4009433E-8136.111117
AGGTCGT551.1441443E-9133.636356
GGTCGTT602.0972948E-9122.57
CCGATCC302.4048127E-4122.5245
CAAGCTA501.01274054E-7122.54
GCAAAAG10950.0117.465763
AAGCAGG13100.0114.083977
CGAAAGC1750.0111.999994
AAGCTAA551.7878665E-7111.363635
ACAAGGT901.8189894E-12108.888893
CAAAAGC12050.0108.775934
AGCAGGT6400.0105.2734458
AGCAAAA13000.098.9423142
AGCAGGG6650.097.631588
Produced by FastQC (version 0.11.2)