FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_72.35200000056e4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_72.35200000056e4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194420
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC20361.047217364468676No Hit
TATTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT10750.5529266536364571No Hit
GTATTAATAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC10160.5225799814833865No Hit
GGCAATCATGGAGAAAAACATCATGTTAAAAGCGAATTTCAATGTGATTT9950.5117786235983952No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG7820.4022219936220554No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC7520.3867914823577821No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC6930.35644481020471147No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG5480.28186400576072423No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCGAAC5440.27980660425882115No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA5170.26591914412097517No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT5100.2623186914926448No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA5030.2587182388643144No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4680.2407159757226623No Hit
ATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAATG4420.22734286596029213No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3950.2031683983129308No Hit
GTAGTTGTAGTGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT3890.20008229606007616No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA3850.19802489455817301No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA3850.19802489455817301No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3840.19751054418269726No Hit
CATATTTTTAGCAAGATCTGCACTAATATTGAGAGGATCAGTTGCTCACA3780.19442444192984262No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3650.18773788704865754No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3580.18413743442032712No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3530.18156568254294825No Hit
GTGATTTTTGGCCGACTAGAGACCATAGTATTAATAAGGGCTTTCACCGA3530.18156568254294825No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3510.1805369817919967No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAGTGCTTGCATGAATGTTATTT3490.17950828104104516No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGA3380.17385042691081165No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3300.16973562390700545No Hit
GCCTTCTTTTCCAGGACATACTATTGAGGATGTCAAAAATGCAATTGGGG3140.1615060178993931No Hit
GTTGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAGAAT3090.1589342660220142No Hit
GCTTCGCGATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTG3030.15584816376915955No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC2990.15379076226725646No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2950.15173336076535338No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2950.15173336076535338No Hit
AAATCTCACCATTGCCTTCTTTTCCAGGACATACTATTGAGGATGTCAAA2940.1512190103898776No Hit
GAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGCAATCATGGAGA2830.14556115625964405No Hit
GTTGTAGTGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT2820.1450468058841683No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2720.13990330212941057No Hit
ATATAAATAAAACAGGGACATTTGAATTCACTAGCTTTTTTTATCGATAT2710.1393889517539348No Hit
TCTTATCTCTTCGAACTTCTGACCTAGCTGTTCTCGCCATTTCCCGTTTC2680.13784590062750746No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2670.13733155025203167No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2610.13424544799917704No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2520.12961629461989507No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC2490.12807324349346777No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCGAACTTCTGACCTA2450.12601584199156465No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2440.12550149161608887No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2410.12395844048966155No Hit
CCATAGTATTAATAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2380.12241538936323423No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2340.12035798786133114No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2340.12035798786133114No Hit
CAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAAGTGGAAGGACCTCTT2320.1193292871103796No Hit
GGTGGCTTTACATTTAAAAGAACAAGCGGGTCGTCAGTCAAAAAAGAAGA2320.1193292871103796No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT2300.11830058635942806No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT2230.11470013373109762No Hit
GGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAACATGATGT2210.11367143298014606No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA2210.11367143298014606No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT2200.11315708260467029No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAGTGCTTGCATGAATGT2200.11315708260467029No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT2190.11264273222919452No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGTCCTTGATTGGG2160.1110996811027672No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2110.10852792922538834No Hit
ATCTTATCTCTTCGAACTTCTGACCTAGCTGTTCTCGCCATTTCCCGTTT2030.10441312622158214No Hit
CTATAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT2020.10389877584610636No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2010.10338442547063059No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGT150.004963807163.33333245
AGAACGA150.004963807163.33333245
GTAACCG650.0150.769234
CGAAAGC1750.0147.000023
GGGTTAA251.1632502E-4147.01
AGCGAAA1800.0142.916671
TAACCGT700.0140.05
CAGGGTG1650.0126.212129
GTATCTA2550.0124.901969
AAAAGCC501.01215846E-7122.53
GCAGGGT1650.0118.787888
GAATCAC3100.0106.693541
GGTCGTT354.439707E-4105.07
ATCACAG3550.093.169023
TGACGCC552.3935065E-589.09091245
CACAGTA3600.088.472225
AGGGTGT1152.3646862E-1185.217396
GTTAATA450.00120531981.666673
GGTTAAT450.00120531981.666672
ACAAGGT903.5037374E-881.666673
Produced by FastQC (version 0.11.2)