FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_63.35200000056dc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_63.35200000056dc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194315
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC23021.1846743689370351No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA11300.5815299899647479No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC10740.5527108046213622No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT9170.47191415999794145No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG9110.4688263901397216No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8820.45390216915832543No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT8710.4482412577515889No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG7110.3659007281990583No Hit
ATTAAAAAGACCACCATGTTACAGCCAAAGGTGATACCATCACTCTATCT6910.3556081620049919No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6490.3339937729974526No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5740.2953966497697038No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4730.24341919048966884No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG4700.24187530556055886No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGA4370.22489257134034943No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT4290.2207755448627229No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4210.21665851838509634No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG4190.21562926176568972No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4070.20945372204924992No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3810.1960733859969637No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3650.18783933304171063No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG3320.17085659882150117No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3220.165710315724468No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3200.16468105910506137No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGA3190.16416643079535806No Hit
GGGTACACTGTTCGAGAAATTTTTCCCTAGTAGTTCATATAGGAGACCGA3160.1626225458662481No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC3100.15953477600802818No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3050.1569616344595116No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC3050.1569616344595116No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT3030.155932377840105No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA3010.15490312122069835No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3010.15490312122069835No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3000.15438849291099505No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2990.1538738646012917No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2870.1476983248848519No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2810.14461055502663203No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2780.14306667009752208No Hit
ATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTTGTTCAT2760.14203741347811544No Hit
GATCTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATC2750.1415227851684121No Hit
ATTAGCACATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGAT2720.13997890023930215No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA2720.13997890023930215No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT2690.1384350153101922No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2640.13586187376167563No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2610.13431798883256568No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2590.13328873221315907No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2580.13277410390345573No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2560.1317448472840491No Hit
GTTCATTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAAGAC2450.12608393587731262No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC2450.12608393587731262No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA2420.12454005094820267No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2420.12454005094820267No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2370.12196690939968607No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2370.12196690939968607No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2350.12093765278027943No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2340.12042302447057612No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG2280.11733525461235624No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2280.11733525461235624No Hit
GAGTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCCTTTTG2240.11527674137354296No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2220.11424748475413633No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2210.113732856444433No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2210.113732856444433No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC2210.113732856444433No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2190.11270359982502637No Hit
ATTAGCACATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTATC2140.11013045827650979No Hit
CACTCTATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTT2130.10961582996680648No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA2080.1070426884182899No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2060.10601343179888327No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2040.10498417517947661No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA2020.10395491856006998No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA1980.10189640532125673No Hit
CCATAGAGTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCC1960.10086714870185008No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC1960.10086714870185008No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1950.10035252039214677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3050.0152.622961
CGAAAGC3300.0141.060614
TAACCGT352.5319605E-6140.05
GTAACCG352.5319605E-6140.04
CAAAAGC12250.0139.04
AAGCAGG15800.0136.455697
AGCAGGT11350.0132.753318
GCAGGGT1700.0122.59
GCAGGTC7200.0119.097239
AGCAAAA14300.0118.21682
GCAAAAG14300.0118.21683
GCGAAAG4000.0116.3753
AGGGTGT950.0116.052636
AGCGAAA4050.0114.938272
ACGGATA1300.0113.076923
GAGCAAA15750.0107.3333361
GCAGGGG801.2551027E-10107.187519
GCAGGTA6100.0104.426239
CGGATAA1450.0101.379314
AAAAGCA16800.0101.3541645
Produced by FastQC (version 0.11.2)