FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_262.35200000056df8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_262.35200000056df8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214322
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA14920.6961487854723267No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT8610.40173197338584005No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT7080.33034406173887887No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6900.32194548389805994No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5740.26782131559056No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4630.21603008557217643No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4610.2150969102565299No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4310.20109928052183165No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4250.198299754574892No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4160.19410046565448252No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3850.17963624826196098No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3660.17077108276331876No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3640.1698379074476722No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3640.1698379074476722No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3640.1698379074476722No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT3590.1675049691585558No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3580.16703838150073255No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG3480.1623725049224998No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGGATCTAATGTCGCA3450.16097274194902997No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3290.15350733942385758No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3150.1469751122143317No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3070.1432424109517455No Hit
GTATACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT3050.14230923563609896No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3010.14044288500480584No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2940.13717677140004292No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2930.13671018374221966No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG2810.13111113184834036No Hit
TAATTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT2810.13111113184834036No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2800.13064454419051708No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2780.1297113688748705No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2650.12364572932316795No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2630.12271255400752139No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2540.11851326508711191No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2530.11804667742928864No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2510.1171135021136421No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2460.11478056382452573No Hit
TAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGA2420.11291421319323261No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT2350.10964809958846969No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2280.10638198598370675No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2260.1054488106680602No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2220.1035824600367671No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2200.10264928472112056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT150.0049641603163.33333245
CGAGGGT150.0049641603163.33333245
GCAGGGG1850.0152.29739
GCTTATA251.1634151E-4147.02
GCAGGGT3100.0142.258069
GCAAAAG12800.0136.855473
AAGCAGG16350.0129.617747
CAAGCTA404.92091E-6122.500014
AAGCTAA404.92091E-6122.500015
TACATCG704.3655746E-11122.55
ATTACAC602.0954758E-9122.499993
AGCAGGT8300.0118.072298
CAAAAGC15600.0113.862184
CGAAAGC3250.0113.076914
GAGCAAA15750.0112.01
CTAAATT458.837587E-6108.888898
AGCAGGG6700.0107.873138
GAGCGAA3450.0106.5217361
GCAGGTA7600.0106.381589
TAGGCAT354.4403342E-4105.09
Produced by FastQC (version 0.11.2)