FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_261.35200000056d71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_261.35200000056d71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190029
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11600.6104331444147998No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG8060.42414578827442123No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT6220.3273184619189703No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC4570.2404896094806582No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT4460.23470101931810405No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4360.22943866462487306No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4040.21259912960653374No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCA4000.2104941877292413No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3410.17944629503917822No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3320.1747101758152703No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3180.16734287924474686No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTGTTTGGGTCTCCATTCCC2980.15681816985828478No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2940.15471322798099238No Hit
GTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCCTGCACCATCTG2890.15208205063437685No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG2890.15208205063437685No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2760.14524098953317652No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2730.1436622831252072No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT2730.1436622831252072No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2680.14103110577859168No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2630.13839992843197615No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2550.13419004467739135No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATGGACGCATTTGAAAAAAAGACG2490.13103263186145273No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2440.12840145451483723No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC2410.12682274810686792No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2360.12419157076025238No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT2330.12261286435228307No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2320.12208662888295997No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG2280.11998168700566757No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2150.11314062590446722No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2040.10735203574191308No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA1930.10156344557935894No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT1910.10051097464071274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAAG8650.0133.121383
GCAGGGT4250.0123.9411859
AGCAAAA9600.0119.9479142
CGAAAGC2150.0108.255824
GAGCAAA10700.0106.471961
CAAAAGC10900.0105.6422044
GCTTATA354.43955E-4105.02
AGCAGGG6800.0102.683828
TCCAACT1051.0913936E-1193.333332
CAACTCT1051.0913936E-1193.333334
GCAGGGG1600.091.875019
CTAAATT552.3933662E-589.090918
CAAGCTA552.3933662E-589.090914
AAGCAGG15800.086.835447
AGGGTGT1351.8189894E-1281.666676
TCTAAAC450.001205276681.666673
TCATAAT450.001205276681.666672
GCAGGTA5550.081.6666649
GCGAAAG2900.080.258623
GAGCGAA2900.080.258621
Produced by FastQC (version 0.11.2)