FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_260.35200000056c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_260.35200000056c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217329
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC10040.4619724012902097No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT9150.4210206645224522No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA5190.2388084424996204No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA4280.19693644198427268No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG4140.19049459575114228No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4050.186353408886987No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGATACTGGGCCATAAGG4040.18589327701319197No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3810.1753102439159063No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT3800.17485011204211126No Hit
TCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT3660.16840826580898086No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3630.16702787018759577No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC3450.15874549645928523No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT3400.15644483709031007No Hit
ATATACAGAAGAGTCGATGGGAAATGGATAAGGGAACTCGTCCTTTATGA3330.15322391397374488No Hit
AGCTTATATAGGCATGAGATTGATGTCCGCCCCATCAGGCCATGAGCCTG3320.15276378209994984No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT3210.14770233148820452No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT3210.14770233148820452No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA3170.1458618039930244No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA3120.14356114462404926No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT3060.14080035338127908No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCTTACCTGCCTGTGCGT3050.14034022150748404No Hit
CTCTTGAACTGAGAAGCGGATACTGGGCCATAAGGACCAGGAGTGGAGGA3040.139880089633689No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT3030.139419957759894No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2990.13757943026471386No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2960.13619903464332878No Hit
ATACAGAAGAGTCGATGGGAAATGGATAAGGGAACTCGTCCTTTATGACA2930.1348186390219437No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2920.13435850714814865No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2920.13435850714814865No Hit
TACTAGCACATTGTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCT2840.13067745215778842No Hit
TTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACACGT2830.13021732028399338No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2740.12607613341983812No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAAACGCATTTGAAAAAAAGACG2700.12423560592465802No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2670.12285521030327293No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2670.12285521030327293No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2660.1223950784294779No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2620.12055455093429776No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2580.11871402343911766No Hit
CTTGATAACTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCC2520.11595323219634748No Hit
TCTTTAGACCATGAAACAATACTATCTACAAGCCTCCCATTGTAAATGAA2520.11595323219634748No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG2510.11549310032255243No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2490.1145728365749624No Hit
GTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGCGTTTGTATCAATG2450.11273230907978225No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2410.11089178158460214No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2390.10997151783701208No Hit
CTTCCTGACAATGTGCTAGTATGAACGATTTTCCCCTCCTCAATGAATAG2390.10997151783701208No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2380.10951138596321705No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2280.10491006722526677No Hit
GTAGTAATGACTGATGGAGGAAAAGCTGATACTAAAATACTATTCATTGA2230.1026094078562916No Hit
GTTAATAATCACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGCAC2230.1026094078562916No Hit
GAATAGACCCTTTCAAACTACTTCAAAACAGCCAAATATACAGCTTAATC2200.10122901223490652No Hit
CTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCCCCCCAAAC2200.10122901223490652No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA204.7810023E-5183.74998245
GGCTTCC150.0049642078163.33333245
GCAGGGG301.1757493E-6163.333339
AAGCAGG3150.0104.999997
AGCAGGT1850.092.7027058
AGCTTAT801.5481419E-891.874991
GCAGGGT852.3581379E-886.47069
CCAACTC1450.084.482763
TAAACTA1051.0895747E-981.6666645
CAAGGGT1254.9112714E-1178.3999948
TCCAACT1600.076.562492
CAACTCT1650.074.2424244
AGGGTAT1001.4761099E-873.56
TTATATT1007.277595E-873.52
ACAAGGG1359.640644E-1172.59267
GGTAAAC852.385099E-672.058833
GCTTATA1203.1377567E-971.458332
GAGCGAA1401.3460522E-1070.01
TAGGCAT1254.3346517E-968.69
CGAAAGC1451.8371793E-1067.586214
Produced by FastQC (version 0.11.2)