FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_241.35200000056db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_241.35200000056db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151188
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC9040.5979310527290526No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT6420.42463687594253513No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5280.34923406619572983No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5070.33534407492658147No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC4440.29367410111913644No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4250.28110696616133557No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC3900.257956980712755No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3880.25663412440140754No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3840.2539884117787126No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3830.25332698362303885No Hit
CTTTTTCAATCTGATGGAATTTCTCGTTGGTTTTCCCGATCAATCGATTC3830.25332698362303885No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3660.24208270497658546No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3640.24075984866523797No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3640.24075984866523797No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG3620.2394369923538905No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC3620.2394369923538905No Hit
GGATTCTCATTAGGTCTGATTAAGCTGTATATTTGGCTATTTTGAAGTAG3530.23348413895282694No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3490.23083842633013202No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3240.21430272243828877No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3170.20967272534857265No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2930.1937984496124031No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG2680.17726274572055983No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2670.1766013175648861No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2630.17395560494219117No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2590.17130989231949625No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2520.16667989522978013No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG2420.16006561367304284No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG2420.16006561367304284No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2390.15808132920602164No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2350.15543561658332672No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2320.15345133211630552No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT2220.14683705055956822No Hit
GTACCCGCTTCTCAGTTCAAGAGTGCTCGATCCCATATTATCCATGTTCT2150.1422070534698521No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2070.13691562822446224No Hit
GTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCGTAGAA2030.13426991560176732No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1820.12037992433261899No Hit
GCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAAC1810.11971849617694524No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT1790.11839563986559778No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC1760.11641135539857661No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC1740.11508849908722914No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT1740.11508849908722914No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1740.11508849908722914No Hit
GCTTCCATTTGGAGTGATGCATTCAGACTTGCATTTGCCAATGGGTGCAT1720.11376564277588168No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA1700.11244278646453422No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1700.11244278646453422No Hit
CTCCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAAC1680.11111993015318676No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1670.11045850199751302No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1670.11045850199751302No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1660.1097970738418393No Hit
GCTTCCCATTGATTTGATCAATTGCTGCTTGAGTGCTTTTGAGATCTGCT1650.10913564568616556No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1630.1078127893748181No Hit
ACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGAC1590.1051670767521232No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1580.10450564859644945No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT1570.10384422044077574No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC1550.10252136412942825No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT1520.10053707966240708No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1520.10053707966240708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAAAT550.0178.181827
AGGGTGT452.8194336E-10163.333336
CTAAATT650.0150.769238
AAGCTAA650.0150.769235
CAAGCTA700.0140.000024
AAGGTAG704.3655746E-11122.500015
CAAGGTA704.3655746E-11122.500014
CGGCCTC302.4032181E-4122.49999245
CGAAAGC458.829102E-6108.888883
ACAAGGT801.2551027E-10107.187513
GGTAGTT801.2551027E-10107.187517
AGGTAGT852.0372681E-10100.8823556
TAGTTTT852.0372681E-10100.8823559
AGCTAAA1051.0913936E-1193.3333366
AAGGGTG852.3532266E-886.470595
TAAATTC1152.3646862E-1185.217399
AGTAAGG450.001204796881.6666642
GCAAAAG3100.075.080643
CAAGGGT1007.262497E-873.54
AAGGGAC500.001830329773.55
Produced by FastQC (version 0.11.2)