FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_236.35200000056c8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_236.35200000056c8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110138
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5530.5020973687555612No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3140.2850968784615664No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2800.25422651582560063No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2760.2505947084566635No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2310.20973687555612053No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2110.19157783871143474No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2010.18249832028909188No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG1990.18068241660462328No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1940.17614265739345186No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1850.16797109081334327No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1760.15979952423323468No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1700.15435181317982893No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1630.14799615028418894No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1600.14527229475748607No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1590.14436434291525177No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1580.1434563910730175No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1580.1434563910730175No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT1580.1434563910730175No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1550.14073253554631462No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1470.13346892080844033No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1460.13256096896620603No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1440.13074506528173746No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT1380.12529735422833174No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1370.12438940238609743No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1340.12166554685939458No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1340.12166554685939458No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1300.11803373949045741No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT1280.11621783580598884No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1260.11440193212152026No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG1260.11440193212152026No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1250.11349398027928599No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG1230.11167807659481742No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG1230.11167807659481742No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1230.11167807659481742No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC1180.10713831738364597No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT1160.1053224136991774No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1140.10350651001470883No Hit
GAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATC1140.10350651001470883No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1130.10259855817247453No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1120.10169060633024025No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1120.10169060633024025No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGA100.0014743947245.000021
GCAGGGG450.0217.777769
AGGGTGT351.1998054E-8175.06
CGTAGAT150.0049609006163.33333245
TATTAAG150.0049609006163.33333245
CTTATAT501.0092117E-7122.53
CGAAAGC653.6416168E-9113.076923
TTATATT653.6416168E-9113.076922
ACAAGGG901.8189894E-12108.888883
TATATTC706.0936145E-9105.03
ATATAGT354.4345387E-4105.06
ATATTCA706.0936145E-9105.04
CAGGGTG602.9925468E-7102.0833369
GCAGGGT1200.0102.0833369
CCTTATA501.4883437E-598.02
GCAAAAG3950.096.139243
CAAGGGT903.1832315E-1095.277774
TATAGTC407.540536E-491.875017
GCTCCCC552.3888802E-589.090917
AAGGGTG707.4936725E-787.55
Produced by FastQC (version 0.11.2)