FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_230.35200000056c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_230.35200000056c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215647
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA14190.6580198194271194No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG14130.6552374946092456No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA10780.49989102561129994No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG10670.49479009677853164No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC10540.48876172633980536No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA9210.427086859543606No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8170.37885989603379594No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC7500.3477906022342068No Hit
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT7370.34176223179548054No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC7360.34129851099250164No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA6420.29770875551248105No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6320.2930715474826916No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA5450.25272783762352363No Hit
ATAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGC5350.2480906295937342No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG5320.24669946718479738No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT5030.23325156389840804No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5020.2327878430954291No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4640.2151664525822293No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC4560.21145668615839774No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA4550.2109929653554188No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4380.20310971170477676No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4370.20264599090179786No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT4290.19893622447796627No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG4270.1980087828720084No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA4250.19708134126605054No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC4240.1966176204630716No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4140.19198041243328215No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4060.18827064600945065No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4010.18595204199455592No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC3950.18316971717668226No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA3900.18085111316178756No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3780.17528646352604024No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATAGTAT3730.17296785951114552No Hit
AGCTTATATAGGCATGAGATTGATGTCCGCCCCATCAGGCCATGAGCCTG3670.17018553469327188No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC3600.1669394890724193No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA3420.1585925146187983No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG3360.15581018980092465No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT3350.1553464689979457No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT3270.15163670257411418No Hit
GTCAAGAAAACTGCTCCTTATTGTTCAGGCTCTTAGGGACAAGCTCGAAC3270.15163670257411418No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG3250.1507092609681563No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3240.15024554016517735No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3180.1474632153473037No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT3180.1474632153473037No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT3170.14699949454432476No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3150.14607205293836686No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT3140.14560833213538793No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3090.14328972812049323No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT3090.14328972812049323No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA3050.14143484490857744No Hit
CTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA3000.13911624089368274No Hit
GATTTCCATTTCATCAATAGTATTCAATAGCCTGTATGCATCACCACAAT2990.1386525200907038No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2980.13818879928772484No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2980.13818879928772484No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA2980.13818879928772484No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT2960.13726135768176695No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG2880.13355159125793542No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2870.13308787045495646No Hit
GTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGC2870.13308787045495646No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2870.13308787045495646No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2860.13262414965197752No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG2860.13262414965197752No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2830.13123298724304072No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2720.12613205841027234No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2720.12613205841027234No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2610.12103112957750398No Hit
GCATTATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCAAT2590.12010368797154611No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA2570.1191762463655882No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA2550.11824880475963032No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2550.11824880475963032No Hit
TTATTGTTCAGGCTCTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTT2540.11778508395665138No Hit
ATAATGCAGTGATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGT2500.11593020074473562No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT2480.11500275913877772No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC2470.11453903833579877No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2440.11314787592686196No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2430.11268415512388301No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA2420.11222043432090407No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2380.1103655511089883No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2380.1103655511089883No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2380.1103655511089883No Hit
CATCAATAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTT2370.10990183030600935No Hit
TCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCA2360.10943810950303041No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2340.10851066789707253No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2340.10851066789707253No Hit
CTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGGACCAGGAGTGGAGGA2310.10711950548813572No Hit
GATGCAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAATATCATTGGG2300.10665578468515677No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA2300.10665578468515677No Hit
AGATACGGACCAGCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGG2290.1061920638821778No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2290.1061920638821778No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2280.10572834307919887No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT2270.10526462227621992No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT2270.10526462227621992No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2260.10480090147324099No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2250.10433718067026204No Hit
CATGTATTCAGATTTCCATTTCATCAATAGTATTCAATAGCCTGTATGCA2180.10109113504940945No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA2180.10109113504940945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA750.0179.666671
AGCGAAA2600.0164.903841
GTTCGGA150.00496418163.33333245
AAGGTAG652.3646862E-11131.923085
CGAAAGC3400.0126.1029363
AGCTTAT1200.0122.51
GGTAGTT801.2551027E-10107.187497
AAGCAGG7900.0106.9936757
AGCAGGT5600.0102.81258
GGACAGT2750.0102.4545446
GCTTATA1500.098.02
TTTAAAC3500.094.500013
GCGAAAG4550.094.230772
GTAGAAA4550.094.230771
GACAGTA3000.093.9166647
TAAACTA3500.091.000015
AGGTAGT954.9294613E-1090.263156
AAGGTCG1550.086.935499
TAGAAAC4800.086.7708362
AGTAGAA2700.086.2037051
Produced by FastQC (version 0.11.2)