FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_208.35200000056deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_208.35200000056deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85953
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACTTGTAAGGGGCAATTCTCCTGTATTCAACTACAACAAGACCACT8390.9761148534664292No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5370.6247600432794667No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA4930.5735692762323595No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4890.568915570137168No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4830.5619350109943806No Hit
GCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTTAATGCTGG4640.5398299070422208No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG3410.39672844461508033No Hit
ATGGCAATGAAATATCCAATCACTGCTGACAAAAGGATAACAGAAATGCT3260.379277046758112No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2750.31994229404441965No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG2090.24315614347375894No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2000.2326853047595779No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1860.21639733342640743No Hit
ATTAGCACAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCT1740.20243621514083276No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1650.1919653764266518No Hit
ATACTTGTAAGGGGCAATTCTCCTGTATTCAACTACAACAAGACCACTAA1560.18149453771247076No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT1470.17102369899828976No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1430.1663699929030982No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1360.15822600723651298No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC1350.1570625807127151No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1350.1570625807127151No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1340.1558991541889172No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1290.15008202156992775No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTC1260.1465917419985341No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1250.1454283154747362No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG1190.13844775633194886No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC1180.13728432980815097No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1120.13030377066536364No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG1070.12448663804637418No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1060.12332321152257629No Hit
GCACATTAGCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTT1050.1221597849987784No Hit
GCCCAGCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTTAAT1040.12099635847498051No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA1020.11866950542738473No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1020.11866950542738473No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC1020.11866950542738473No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC980.11401579933219318No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCATTGCTATAACTATTTTAA980.11401579933219318No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA960.1116889462845974No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC950.11052551976079951No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT920.10703524018940583No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC900.10470838714181005No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA900.10470838714181005No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG870.10121810757041638No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC870.10121810757041638No Hit
CATCCCTGATCCCCTCACATTCACAGTCAGTGAAGAGAACTGCATTCTGC860.1000546810466185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC402.6589987E-8153.124981
AGGGTGT251.1610097E-4147.06
TAGATGG251.1610097E-4147.08
CGAAAGC850.0129.705893
TCGTACT501.00730176E-7122.52
GCAGGGT501.00730176E-7122.59
CAAGGGT404.9040063E-6122.499994
CAAGCTA458.807279E-6108.888894
GCGAAAG1050.0105.000012
AAGGGTG354.431193E-4105.05
CCAACCC354.431193E-4105.08
GCAAAAG2900.0101.379313
CAAAAGC3150.0101.1111154
ACAAGGG501.4864752E-598.03
AAGCTAA501.4864752E-598.05
TTTAAAC1500.089.8333363
CGTACTC1007.3305273E-1085.753
AAGGTAG450.001203017481.666675
CAGTACG450.001203017481.666679
GGTAGTT450.001203017481.666677
Produced by FastQC (version 0.11.2)