FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_202.35200000056e13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_202.35200000056e13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182288
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA12760.6999912226805933No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT5030.27593697884666024No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4270.23424471166505748No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4120.2260159747213201No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3820.20955850083384533No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3530.19364960940928638No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3470.19035811463179145No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3410.1870666198542965No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3300.18103221276222242No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3130.17170631089265337No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3050.1673176511893268No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3030.16622048626349514No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2890.15854033178267357No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2820.1547002545422628No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2770.15195734222768367No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2670.1464715175985254No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2620.1437286052839463No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2490.13659703326604056No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTAACTAAGGG2460.13495128587729308No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2450.13440270341437724No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2400.1316597910997981No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2380.13056262617396647No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2350.128916878785219No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2280.1250768015448082No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2250.12343105415606073No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2240.12288247169314492No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2230.12233388923022909No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2230.12233388923022909No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2120.11629948213815501No Hit
GCGTGAAAACAAACCCCAAAATCCCCTTAGTTAAAGGTGACAGAATTGGT2090.11465373474940754No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2010.11026507504608093No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1970.10807074519441763No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1960.1075221627315018No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC1950.10697358026858597No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1950.10697358026858597No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1940.10642499780567016No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG1870.10258492056525936No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT1830.10039059071359607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA100.0014751855245.00002245
GCTAAAT301.1752327E-6163.333337
CTAAATT352.5315348E-6140.08
AGCAGGT5550.0119.189188
GCAGGGG950.0116.052639
CAGGGTG551.7864113E-7111.363649
AAGCTAA458.834015E-6108.888895
AAGCAGG9550.0107.7486957
GCAAAAG7750.0107.483873
CGAAAGC1950.0106.7948764
GTGTGAA354.439257E-4105.01
AGGGGGG354.439257E-4105.0245
CAAGCTA501.49098E-597.999994
AGCAAAA8800.096.051142
GCAGGGT1600.091.875019
AGGGTTA707.513554E-787.59
CAAAAGC10000.084.5254
GAGCGAA2500.083.31
GTATTAA1051.0895747E-981.666671
GTCGTAC450.001205197281.666671
Produced by FastQC (version 0.11.2)