FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_177.35200000056d64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_177.35200000056d64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200955
Sequences flagged as poor quality0
Sequence length251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA8410.41850165459928845No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC7960.3961085815232266No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC6600.3284317384489065No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG4920.24483093229827574No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT4000.1990495384538827No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC3880.19307805230026623No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC3800.18909706153118858No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA3770.18760418999278447No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA3450.17168022691647383No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA3270.16272299768604911No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC2910.14480853922519968No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC2860.14232041999452616No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT2730.13585130999477496No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2620.13037744768729317No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT2470.12291308999527258No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT2450.12191784230300316No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA2380.11843447538006022No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA2360.1174392276877908No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT2270.11296061307257843No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC2160.10748675076509666No Hit
CAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGGGCCCA2090.10400338384215371No Hit
ATATTGTGTAGAATTAGACCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC2080.103505759996019No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC2050.10201288845761489No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2050.10201288845761489No Hit
AGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGC2030.10101764076534549No Hit
GGCACGTGCCACTGTGCTCAGCTAATTTTTCTATTTTTAGTAGAGATGGG2020.10052001691921078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1750.0161.000021
GAGCAAA8050.0146.086941
CAAAAGC9100.0130.576924
GCAAAAG9650.0123.134723
CGGGATG302.4043856E-4122.55
AGCAAAA10150.0115.8620762
AAAGCAG12800.0115.800776
AAAAGCA10850.0109.516135
AGCGAAA2750.0102.4545442
CATACCG603.0016417E-7102.0833369
CGAAAGC2800.0100.6254
GCGAAAG2800.0100.6253
AGCAGGA5150.099.902918
GCGGGAT407.549678E-491.874994
GCAGGAC2650.083.207549
AAGCAGG18950.076.9261257
AGCAGGG6300.071.944448
CGCACTG903.3482174E-668.05556245
TATCGTG3450.067.463772
ATCGTGT3500.066.500013
Produced by FastQC (version 0.11.2)