FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_174.35200000056cb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_174.35200000056cb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111534
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4190.375670199221762No Hit
GAATCACAGTATCTACCAAAAAAAGCCAACAAGCTGTAATCCCAAATATC4100.367600910932989No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT4040.36222138540714044No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGA3420.30663295497337134No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3140.28152850251941114No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC2840.25463087489016806No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2750.2465615866013951No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2640.2366991231373393No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGAT2620.23490594796205644No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2610.234009360374415No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2410.21607760862158623No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2240.2008356196316818No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2240.2008356196316818No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC2230.1999390320440404No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGATGTGTG2150.19276633134290888No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2130.19097315616762603No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2060.18469704305413595No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1940.17393799200243873No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1870.16766187888894868No Hit
CATCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1860.1667652913013072No Hit
CAAGTTGTAGACAAAAAATTGAGTGATGCCCCATTCCTCGATCGGCTTCG1850.1658687037136658No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1780.15959259060017572No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG1710.15331647748668567No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA1710.15331647748668567No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1700.15241988989904426No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1540.13807448849678125No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1510.13538472573385693No Hit
TAGTAATACTATGGTCTCTAATCGGCCAAAAATCACATTGAAATTCGCTT1470.1317983753832912No Hit
GGATTCTCATTAGGTCTGATTAAGCTGTATATTTGGCTATTTTGAAGTAG1400.12552226226980112No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1370.12283249950687683No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA1350.12103932433159396No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGATGT1350.12103932433159396No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1310.11745297398102822No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAGAATAA1280.1147632112181039No Hit
GGCCTGATGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1270.11386662363046245No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT1260.11297003604282102No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG1260.11297003604282102No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1230.1102802732798967No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC1190.10669392292933097No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1190.10669392292933097No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1180.10579733534168954No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1180.10579733534168954No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1160.10400416016640666No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC1150.10310757257876522No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1140.10221098499112377No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG1130.10131439740348235No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTAA100.0014744196245.000025
TATGCGG150.0049609845163.33333245
CTAAATT150.0049609845163.333338
CAAGCTA150.0049609845163.333334
GCTAAAT150.0049609845163.333337
ACGGATA302.4015426E-4122.53
GCAGGGT602.0863808E-9122.59
GCAGGGG302.4015426E-4122.59
CAAGGTA653.6416168E-9113.076929
AGGGTGT551.7818093E-7111.363646
TACGGAT354.4346892E-4105.02
ACAAGGT706.0954335E-9105.08
TAGAAAC1007.2759576E-1297.999993
CGAAAGC654.821013E-794.230774
AAGCAGG3600.088.472227
TATGATA707.494309E-787.55
AGCAAAA2950.087.203392
TAGACAA1600.084.218758
AGTAAAG450.001203962681.666672
AGCAGGG1550.079.032268
Produced by FastQC (version 0.11.2)