FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_173.35200000056c65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_173.35200000056c65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194819
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT16770.8607989980443386No Hit
TGGACAGTAGTCCCCCAAAGGAGTGGAAGAAGGTTCCATTGGGAAAGTCT14820.7607060912949969No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10360.5317756481657333No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA8100.4157705357280347No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA7960.40858437832038974No Hit
CTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAACTCTTTGGTCA7400.3798397486898095No Hit
GTATTGATTCGCCTTGTTCATTGATGAAATGGAAATCTGAATACATGAAA6260.32132389551327134No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC5920.3038717989518476No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGAAGCCGAGTCCTCAGTT5750.29514575067113574No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5520.28333992064429037No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC5390.27666706019433424No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA4750.2438160549022426No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4710.2417628670714869No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4680.24022297619842006No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4590.2356033035792197No Hit
ATACTTGACGATCCAAATGCACTGTTAAAGCACAGATTTGAAATAATCGA4420.22687725529850783No Hit
GTCTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGCTCCTTATTGTTCAG4080.20942515873708417No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA3730.19145976521797156No Hit
GTCAAGTTCTACTACTATTGATTCGCCTTGTTCATTGATGAAATGGAAAT3580.18376031085263758No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3450.17708745040268145No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3440.17657415344499253No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC3430.1760608564873036No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3410.17503426257192575No Hit
AATCAATAGTAGTAGAACTTGACGATCCAAGGCTAAGTCAGTATTCAATA3380.17349437169885895No Hit
AAGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGC3370.17298107474117No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3270.1678481051642807No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3230.16579491733352497No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3210.1647683234181471No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3120.16014865079894672No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3110.15963535384125777No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3050.1565555720951242No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA2850.14628963294134556No Hit
CTTCCAATTGTGGTGATGCATACACTGACTTAGCCAATAGAGTCCTACAG2820.14474974206827876No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT2820.14474974206827876No Hit
TGCATACAGGCTATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTT2760.14166996032214518No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA2630.13499709987218905No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2600.13345720899912225No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2560.13140402116836655No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA2530.12986413029529975No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2500.12832423942223295No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2440.12524445767609935No Hit
ATTGTTCTGTCTCTCCCCTCGATTATTTCAAATCTGTGCTTTAACAGTGC2430.12473116071841042No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG2430.12473116071841042No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2420.12421786376072148No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2410.12370456680303255No Hit
GTCTGTAGGACTCTATTGGCTAAGTCAGTGTATGCATCACCACAATTGGA2410.12370456680303255No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2380.12216467592996577No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2370.12165137897227683No Hit
GTCTGTAGGACTCTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATC2370.12165137897227683No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2360.1211380820145879No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2340.12011148809921003No Hit
ATAGTAGTAGAACTTGACGATCCAAGGCTAAGTCAGTATTCAATAGCCTG2280.11703170635307643No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2270.11651840939538752No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGGCG2250.11549181548000965No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA2240.11497851852232072No Hit
CTACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGATTATTTCAAATCTG2210.11343862764925391No Hit
CCTTCCAGACTTTCCCAATGGAACCTTCTTCCACTCCTTTGGGGGACTCC2160.11087214286080926No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2150.11035884590312034No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2060.10573917328391995No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGAA2040.10471257936854209No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2000.10265939153778635No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1990.10214609458009744No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC1980.10163279762240848No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA1970.10111950066471957No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1950.1000929067493417No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGG150.0049638157163.333337
TAACCGT150.0049638157163.333335
CAAGGTG150.0049638157163.333335
GCAGGGG1350.0163.333339
GTAACCG150.0049638157163.333334
GCAAAAG9100.0137.30773
GCAGGGT1400.0131.259
AAGCAGG12350.0126.963577
GAGCGAA2650.0124.811321
CAAAAGC10200.0122.499994
AGCAGGT8400.0121.041678
AGCAGGG3450.0117.173918
GCAGGTA8150.0115.736199
AGGGTGT851.8189894E-12115.294116
GCGAAAG2900.0114.051723
GGTAGTT551.786866E-7111.363647
AGTAGTC3300.0100.227276
AGCGAAA3500.094.500012
CGACGAA407.549326E-491.87501245
AAAGCAG17250.090.898556
Produced by FastQC (version 0.11.2)