FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_17.35200000056d21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_17.35200000056d21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208039
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT5310.2552406039252256No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA4570.21967035027086268No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT3840.1845807757199371No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3650.17544787275462775No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA3310.1591047832377583No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG3250.15622070861713427No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT3180.15285595489307294No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2980.1432423728243262No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2940.14131965641057687No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2920.1403582982037022No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA2850.13699354447964082No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2840.1365128653762035No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2770.13314811165214213No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2700.1297833579280808No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2690.12930267882464347No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2690.12930267882464347No Hit
GTACGTGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT2660.12786064151433146No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2640.12689928330745676No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2510.1206504549627714No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2510.1206504549627714No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2450.11776638034214738No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG2450.11776638034214738No Hit
AGCTTATATAGGCATGAGATTGAGGTCCGCCCCATCAGGCCATGAGCCTG2380.11440162661808605No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG2370.11392094751464871No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2360.11344026841121135No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT2330.11199823110089936No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTACCTGTGCAT2320.11151755199746202No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2130.10238464903215264No Hit
GGGTATGAAACCTTCAAAGTCGTTGAAGGCTGGTCCAACCCTAAGTCCAA2130.10238464903215264No Hit
CTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGGACCAGGAGTGGAGGA2120.1019039699287153No Hit
GTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTTCTGCATTGTC2110.10142329082527796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG1400.0122.52
CGAAAGC1550.0110.645163
AGCGAAA1550.0110.645161
GTCGTAC1600.091.874991
AAGGGTA552.3939025E-589.0909049
CGTACTC1700.086.470593
TCGTACT1800.081.666672
ACGAGTA550.002672489766.818182
ACAAGGT954.615713E-664.473693
CGAGTAC600.003772729561.253
TACTCTT2400.061.255
TCCAACT1852.3646862E-1159.5945932
ATATAGG1253.4082404E-758.86
GTTCTAT650.005179523556.538461
TATAGGC1304.469057E-756.538467
TATATAG2650.055.47175
GTAGAAA2450.055.01
TAGGCAT1355.799666E-754.4444479
GCTTATA1407.454473E-752.52
CTATGCT3000.049.0000044
Produced by FastQC (version 0.11.2)